Department of Chemistry, University of Oxford, Oxford OX1 3QZ, United Kingdom.
Nuffield Department of Clinical Neurosciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom.
Anal Chem. 2020 Oct 20;92(20):13647-13651. doi: 10.1021/acs.analchem.0c03092. Epub 2020 Oct 5.
The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson's disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).
神经元衍生外泌体中α-突触核蛋白的排出先于帕金森病（PD）的临床症状出现，为开发预测或预后测试提供了一种手段。在这里，我们报告了一种无试剂的阻抗测定法，用于测定神经元来源的外泌体中的两种内部外泌体标志物（α-突触核蛋白和衔接蛋白-1）。在裂解之前，使用抗 L1CAM 偶联两性离子聚合物修饰的磁性珠从患者血清中有效地提取外泌体，并通过电化学阻抗谱进行分析。对 40 个临床样本中α-突触核蛋白水平的定量分析显示，PD 患者与健康对照组（HC）之间存在统计学显著差异。
