Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
J Fish Dis. 2012 Oct;35(10):733-9. doi: 10.1111/j.1365-2761.2012.01398.x. Epub 2012 Jul 18.
A second type of freshwater crab reovirus has been isolated from Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, in China; we named it E. sinensis reovirus (EsRV816). The negatively stained virion is a non-enveloped icosahedral particle, 60 ± 5 nm in diameter. Its genome is composed of 10 dsRNA linear pieces exhibiting an electrophoretic pattern of 5/3/2. The largest segment (RNA-1) was cloned and sequenced. The deduced amino acid sequence, corresponding to the RdRp of the virus, showed 26% identity with the RdRp of Operophtera brumata (L.) cypovirus 19 in the genus Cypovirus and 24% identity with RdRp of Nilaparvata lugens (Stal) reovirus in the genus Fijivirus. On the basis of its ultrastructure and physicochemical properties, this virus is quite different from other crab reoviruses, and particularly with another freshwater crab reovirus EsRV905, recently classified in a new genus Cardoreovirus. This virus (EsRV816) possesses all the characters of the members of the reoviridae family and could represent a new genus.
已从中国中华绒螯蟹（Eriocheir sinensis H. Milne Edwards）中分离出第二种淡水蟹呼肠孤病毒，我们将其命名为中华绒螯蟹呼肠孤病毒 816 型（EsRV816）。负染的病毒粒子为无包膜的二十面体，直径 60±5nm。其基因组由 10 个 dsRNA 线性片段组成，电泳图谱呈 5/3/2 型。我们克隆并测序了最大片段（RNA-1）。相应病毒 RdRp 的推导氨基酸序列与 Cypovirus 属的Operophtera brumata（L.） Cypovirus 19 的 RdRp 有 26%的同一性，与 Fijivirus 属的 Nilaparvata lugens（Stal）呼肠孤病毒的 RdRp 有 24%的同一性。基于其超微结构和理化特性，该病毒与其他蟹呼肠孤病毒明显不同，特别是与最近在一新属 Cardoreovirus 中分类的另一种淡水蟹呼肠孤病毒 EsRV905 不同。该病毒（EsRV816）具有呼肠孤病毒科成员的所有特征，可代表一个新属。
