Graduate Program in Zoology of the Federal University of Paraná, Curitiba, Paraná 80035-050, Brazil; Integrated Group of Aquaculture and Environmental Studies, Federal University of Paraná, Curitiba, Paraná 80035-050, Brazil.
Biotechnology Center, Cellular and Molecular Biotechnology Laboratory, Federal University of Paraíba, João Pessoa, Paraíba 58051-900, Brazil.
J Invertebr Pathol. 2024 Jun;204:108118. doi: 10.1016/j.jip.2024.108118. Epub 2024 Apr 27.
Portunid crabs are distributed worldwide and highly valued in aquaculture. Viral infections are the main limiting factor for the survival of these animals and, consequently, for the success of commercial-scale cultivation. However, there is still a lack of knowledge about the viruses that infect cultured portunid crabs worldwide. Herein, the genome sequence and phylogeny of Callinectes sapidus reovirus 2 (CsRV2) are described, and the discovery of a new bunyavirus in Callinectes danae cultured in southern Brazil is reported. The CsRV2 genome sequence consists of 12 dsRNA segments (20,909 nt) encode 13 proteins. The predicted RNA-dependent RNA polymerase (RdRp) shows a high level of similarity with that of Eriocheir sinensis reovirus 905, suggesting that CsRV2 belongs to the genus Cardoreovirus. The CsRV2 particles are icosahedral, measuring approximately 65 nm in diameter, and exhibit typical non-turreted reovirus morphology. High throughput sequencing data revealed the presence of an additional putative virus genome similar to bunyavirus, called Callinectes danae Portunibunyavirus 1 (CdPBV1). The CdPBV1 genome is tripartite, consisting of 6,654 nt, 3,120 nt and 1,656 nt single-stranded RNA segments that each encode a single protein. Each segment has a high identity with European shore crab virus 1, suggesting that CdPBV1 is a new representative of the family Cruliviridae. The putative spherical particles of CdPBV1 measure ∼120 nm in diameter and present a typical bunyavirus morphology. The results of the histopathological analysis suggest that these new viruses can affect the health and, consequently, the survival of C. danae in captivity. Therefore, the findings reported here should be used to improve prophylactic and pathogen control practices and contribute to the development and optimization of the production of soft-shell crabs on a commercial scale in Brazil.
梭子蟹分布广泛，在水产养殖中具有很高的价值。病毒感染是这些动物生存的主要限制因素，也是商业规模养殖成功的关键。然而，目前人们对全球养殖梭子蟹所感染的病毒仍知之甚少。本文描述了美洲蓝蟹呼肠孤病毒 2 型（CsRV2）的基因组序列和系统发育，并报告了在巴西南部养殖的万氏对虾呼肠孤病毒在 Callinectes danae 中的发现。CsRV2 基因组序列由 12 个 dsRNA 片段（20909nt）组成，编码 13 种蛋白。预测的 RNA 依赖性 RNA 聚合酶（RdRp）与中华绒螯蟹呼肠孤病毒 905 具有高度相似性，表明 CsRV2 属于呼肠孤病毒科。CsRV2 粒子呈二十面体，直径约 65nm，具有典型的无塔突轮状呼肠孤病毒形态。高通量测序数据显示，存在一种类似布尼病毒的额外推定病毒基因组，称为万氏对虾 Portunibunyavirus 1（CdPBV1）。CdPBV1 基因组为三分体，由 6654nt、3120nt 和 1656nt 单链 RNA 片段组成，每个片段编码一种单一蛋白。每个片段与欧洲滨蟹病毒 1 具有高度的同源性，表明 CdPBV1 是克鲁利病毒科的一个新代表。CdPBV1 的推定球形粒子直径约 120nm，具有典型的布尼病毒形态。组织病理学分析结果表明，这些新病毒会影响 C.danae 的健康，进而影响其在圈养环境下的生存。因此，本研究报告的结果可用于改进预防和病原体控制措施，并有助于巴西商业规模软壳蟹生产的开发和优化。
