Chiappori Alessandra, Folli Chiara, Balbi Francesco, Caci Emanuela, Riccio Anna Maria, De Ferrari Laura, Melioli Giovanni, Braido Fulvio, Canonica Giorgio Walter
Allergy and Respiratory Diseases, IRCCS San Martino-IST-University of Genoa, Genoa, Italy.
Istituto Giannina Gaslini, Genoa, Italy.
World Allergy Organ J. 2016 Feb 12;9:5. doi: 10.1186/s40413-016-0095-2. eCollection 2016.
Chronic obstructive pulmonary disease (COPD) is a progressive lung disorder characterized by poorly reversible airway obstruction and its pathogenesis remains largely misunderstood. Local changes of regulatory T-cell populations in the lungs of COPD patients have been demonstrated although data concerning their pathologic role are contrasting. The aim of our study was to evaluate the relative percentage of regulatory T-cells in the peripheral blood of current and former smoker subjects, affected or not by COPD. Furthermore, the effect of different concentrations of budesonide and formoterol, on regulatory T-cells has been investigated.
T regulatory lymphocytes were isolated and assessed as CD4(+)CD25(high)CD127(-) cells by flow cytometry and cultured for 48 hours in the absence or in the presence of budesonide and/or formoterol at different doses.
CD4(+)CD25(high)CD127(-) regulatory T-cells percentage was significantly reduced in COPD patients, both current and former smokers, with respect to volunteers. Furthermore, CD4(+)CD25(high)CD127(-) cells of COPD patients showed a not statistically significant response to drugs compared to healthy subjects.
Our results evidenced a different behaviour of CD4(+)CD25(high)CD127(-) Treg cells in COPD patients after in vitro treatments.
Based on our data, we suggested a possible role of CD4 CD25(high)CD127 T-cells in COPD pathogenesis.
慢性阻塞性肺疾病（COPD）是一种以气道阻塞可逆性差为特征的进行性肺部疾病，其发病机制在很大程度上仍未被理解。虽然关于COPD患者肺部调节性T细胞群体局部变化的数据存在矛盾，但已有研究证实了这些变化。我们研究的目的是评估当前吸烟者和既往吸烟者外周血中调节性T细胞的相对百分比，无论他们是否患有COPD。此外，还研究了不同浓度的布地奈德和福莫特罗对调节性T细胞的影响。
通过流式细胞术分离并评估调节性T淋巴细胞为CD4(+)CD25(高)CD127(-)细胞，并在不存在或存在不同剂量的布地奈德和/或福莫特罗的情况下培养48小时。
与志愿者相比，当前吸烟者和既往吸烟者中的COPD患者CD4(+)CD25(高)CD127(-)调节性T细胞百分比均显著降低。此外，与健康受试者相比，COPD患者的CD4(+)CD25(高)CD127(-)细胞对药物的反应无统计学意义。
我们的结果证明了体外治疗后COPD患者中CD4(+)CD25(高)CD127(-)调节性T细胞的不同行为。
根据我们的数据，我们认为CD4 CD25(高)CD127 T细胞在COPD发病机制中可能发挥作用。
