Safadi Rima A, Bader Dima H, Abdullah Niveen I, Sughayer Maher A
Associate Professor, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan.
Oral Medicine specialist, Ministry of Health, Amman, Jordan.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 May;121(5):510-9. doi: 10.1016/j.oooo.2015.11.016. Epub 2015 Dec 19.
To investigate the immunohistochemical expression (IHCE) of selected keratins in primary cutaneous and mucosal melanoma (pM), and metastatic melanoma (metsM) of the head and neck and to compare their expression to a group of undifferentiated/poorly differentiated tumors of the same anatomic region.
IHCE of K6, K7, K8, K14, K16, K18, and K19 were studied in 29 melanomas and 70 cases of non-melanoma tumors of the same anatomic region (neuroendocrine carcinoma, neuroblastoma, olfactory neuroblastoma, sinonasal undifferentiated carcinoma, undifferentiated nasopharyngeal carcinoma, anaplastic large cell lymphoma, poorly differentiated squamous cell carcinoma (PDSCC), and Ewing sarcoma). MNF-116 pancytokeratin was investigated in melanoma.
All studied keratins, except K6, were expressed in melanoma. IHCE of MNF-116, K8, and K18 was higher in metsM compared with pM. K14 and K16 expression was highest in PDSCC.
metsM expresses keratins more than pM, specifically K8, K18, and MNF-116. Keratin positivity in an undifferentiated or poorly differentiated neoplasm does not necessarily exclude the diagnosis of melanoma.
研究特定角蛋白在原发性皮肤和黏膜黑色素瘤（pM）以及头颈部转移性黑色素瘤（metsM）中的免疫组化表达（IHCE），并将其表达与同一解剖区域的一组未分化/低分化肿瘤进行比较。
对29例黑色素瘤和70例同一解剖区域的非黑色素瘤肿瘤（神经内分泌癌、神经母细胞瘤、嗅神经母细胞瘤、鼻窦未分化癌、未分化鼻咽癌、间变性大细胞淋巴瘤、低分化鳞状细胞癌（PDSCC）和尤因肉瘤）进行K6、K7、K8、K14、K16、K18和K19的免疫组化研究。在黑色素瘤中研究MNF-116全细胞角蛋白。
除K6外，所有研究的角蛋白均在黑色素瘤中表达。与pM相比，metsM中MNF-116、K8和K18的免疫组化表达更高。K14和K16在PDSCC中的表达最高。
metsM比pM表达更多角蛋白，特别是K8、K18和MNF-116。未分化或低分化肿瘤中的角蛋白阳性不一定排除黑色素瘤的诊断。
