Mohod Smeeta M, Kandhare Amit D, Bodhankar Subhash L
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Paud Road, Erandwane, Pune 411038, Maharashtra, India.
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Paud Road, Erandwane, Pune 411038, Maharashtra, India.
J Ethnopharmacol. 2016 Apr 22;182:150-9. doi: 10.1016/j.jep.2016.02.026. Epub 2016 Feb 21.
Madhuca indica J. F. Gmel. (Sapotaceae) has shown antioxidant, anti-inflammatory, analgesic, anti-diabetic and hepatoprotective potential. It has been traditionally used as laxative, tonic, anti-burn, anti-earthworm, wound healing and headache.
To investigate the efficacy and possible mechanism of Madhuca indica J. F. Gmel. leaves methanolic extract (MI-ALC) and its isolated chloroform fraction (D3) against experimental induced gastric ulcers.
D3 was isolated from MI-ALC, well characterized (HPTLC, FT-IR, (1)H-NMR and LC-MS) and evaluated for its gastroprotective activity by using acetic acid induced ulcer in male Wistar rats (150-200g). D3 (2.5, 5 and 10mg/kg, p.o.) were administered for the period of 14 days. At the end of treatment, rats were sacrificed to collect the stomach sample for evaluation of antioxidant (SOD, GSH, and MDA) enzyme, oxido-inflammatory (TNF-α, IL-1, iNOs) and prostaglandins (COX-II) markers by using RT-PCR.
The structure and molecular weight (MW) of the isolated compound (D3) were confirmed by 1D and 2D spectral data and characterized as 3,5,7,3',4'-Pentahydroxy flavone with MW C15H10O7. Administration of 3,5,7,3',4'-Pentahydroxy flavone (5 and 10mg/kg) significantly and dose-dependently inhibited (P<0.01 and P<0.001) acetic acid induced an alteration in the antioxidant enzyme. It also significantly and dose-dependently down-regulated gastric oxido-inflammatory and prostaglandins markers. Histopathological aberration induced in the stomach also attenuated by 3,5,7,3',4'-Pentahydroxy flavone treatment.
Finding of present investigation suggests that MI-ALC possessed potent antiulcer activity due to the presence of 3,5,7,3',4'-Pentahydroxy flavone via its oxido-inflammatory and prostaglandins modulatory potential.
阔叶树（Madhuca indica J. F. Gmel.）（山榄科）已显示出抗氧化、抗炎、镇痛、抗糖尿病和保肝潜力。它传统上被用作泻药、滋补品、抗烧伤药、抗蚯蚓药、伤口愈合药和治疗头痛的药物。
研究阔叶树叶子甲醇提取物（MI-ALC）及其分离的氯仿部分（D3）对实验性诱导胃溃疡的疗效及可能机制。
从MI-ALC中分离出D3，进行充分表征（高效薄层层析、傅里叶变换红外光谱、氢核磁共振和液相色谱-质谱），并通过在雄性Wistar大鼠（150-200克）中使用乙酸诱导溃疡来评估其胃保护活性。给予D3（2.5、5和10毫克/千克，口服），持续14天。治疗结束时，处死大鼠以收集胃样本，通过逆转录聚合酶链反应评估抗氧化酶（超氧化物歧化酶、谷胱甘肽和丙二醛）、氧化炎症（肿瘤坏死因子-α、白细胞介素-1、诱导型一氧化氮合酶）和前列腺素（环氧化酶-II）标志物。
通过一维和二维光谱数据确认了分离化合物（D3）的结构和分子量（MW），并将其表征为分子量为C15H10O7的3,5,7,3',4'-五羟基黄酮。给予3,5,7,3',4'-五羟基黄酮（5和10毫克/千克）显著且剂量依赖性地抑制（P<0.01和P<0.001）乙酸诱导的抗氧化酶变化。它还显著且剂量依赖性地下调胃氧化炎症和前列腺素标志物。3,5,7,3',4'-五羟基黄酮治疗也减轻了胃中诱导的组织病理学异常。
本研究结果表明，由于存在3,5,7,3',4'-五羟基黄酮，MI-ALC通过其氧化炎症和前列腺素调节潜力具有强大的抗溃疡活性。
