Dessì Angelica, Murgia Antonio, Agostino Rocco, Pattumelli Maria Grazia, Schirru Andrea, Scano Paola, Fanos Vassilios, Caboni Pierluigi
Neonatal Intensive Care Unit, Puericulture Institute and Neonatal Section, Azienda Ospedaliera Universitaria, University of Cagliari, 09042 Monserrato, Italy.
Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Int J Mol Sci. 2016 Feb 22;17(2):265. doi: 10.3390/ijms17020265.
In this study, a gas-chromatography mass spectrometry (GC-MS) metabolomics study was applied to examine urine metabolite profiles of different classes of neonates under different nutrition regimens. The study population included 35 neonates, exclusively either breastfed or formula milk fed, in a seven-day timeframe. Urine samples were collected from intrauterine growth restriction (IUGR), large for gestational age (LGA), and appropriate gestational age (AGA) neonates. At birth, IUGR and LGA neonates showed similarities in their urine metabolite profiles that differed from AGA. When neonates started milk feeding, their metabolite excretion profile was strongly characterized by the different diet regimens. After three days of formula milk nutrition, urine had higher levels of glucose, galactose, glycine and myo-inositol, while up-regulated aconitic acid, aminomalonic acid and adipic acid were found in breast milk fed neonates. At seven days, neonates fed with formula milk shared higher levels of pseudouridine with IUGR and LGA at birth. Breastfed neonates shared up-regulated pyroglutamic acid, citric acid, and homoserine, with AGA at birth. The role of most important metabolites is herein discussed.
在本研究中，采用气相色谱-质谱联用（GC-MS）代谢组学研究方法，检测不同营养方案下不同类型新生儿的尿液代谢物谱。研究对象包括35名在7天时间内完全采用母乳喂养或配方奶喂养的新生儿。从宫内生长受限（IUGR）、大于胎龄（LGA）和适于胎龄（AGA）的新生儿中采集尿液样本。出生时，IUGR和LGA新生儿的尿液代谢物谱相似，与AGA新生儿不同。当新生儿开始喂奶时，他们的代谢物排泄谱因不同的饮食方案而有显著特征。配方奶喂养3天后，尿液中葡萄糖、半乳糖、甘氨酸和肌醇水平较高，而母乳喂养的新生儿中发现乌头酸、氨基丙二酸和己二酸上调。7天时，出生时配方奶喂养的新生儿与IUGR和LGA新生儿的假尿苷水平较高。母乳喂养的新生儿与出生时的AGA新生儿共有上调的焦谷氨酸、柠檬酸和高丝氨酸。本文讨论了最重要代谢物的作用。
