Universidade Estadual de Goiás (UEG), Campus Anápolis de Ciências Exatas e Tecnológicas, Anápolis, GO, Brazil.
Instituto de Física, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Mar 5;210:329-334. doi: 10.1016/j.saa.2018.11.050. Epub 2018 Nov 19.
Recent research has shown that latex from different species is able to produce tissue replacement and regeneration. Particularly, biomembranes obtained from Hancornia speciosa latex (HSB) have shown high angiogenic and osteogenic activity. Considering new materials for wound healing, it would be interesting to develop a product combining antibacterial and antifungal activities. Silver nanoparticles (AgNP) have been commonly used for this purpose in medicinal products and devices for decades. In order to combine angiogenic, antibacterial and antifungal properties on the same platform, we developed an HSB containing 3 concentrations of AgNP. It was observed that the HSB successfully accommodated the AgNP in the matrix and released them in a controlled way. The release dynamics of AgNP by HSB was described by UV-vis absorption spectroscopy. The released nanoparticles were evaluated by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) measurements. In addition, the cytotoxic and genotoxic effects were evaluated using the Allium cepa assay. The results showed no cytotoxic effect of HSB-AgNP in all studied concentrations. The genotoxic effect was observed in HSB-AgNP at the two highest concentrations, however not at the lowest concentration. Thus, the addition of AgNP at the lowest concentration can improve the pharmacological activity of HSB without causing a toxic effect on vegetal cells. Therefore, the H. speciosa latex biomembrane presented in this paper combines angiogenic, anti-inflammatory and antibacterial properties and can be considered potentially new biomaterial for wound-healing.
最近的研究表明，不同物种的乳胶能够产生组织替代和再生。特别是，从木菠萝乳胶（HSB）中获得的生物膜显示出较高的血管生成和成骨活性。考虑到用于伤口愈合的新材料，开发一种结合抗菌和抗真菌活性的产品将是很有趣的。几十年来，银纳米粒子（AgNP）已在药物产品和设备中常用于此目的。为了在同一平台上结合血管生成、抗菌和抗真菌特性，我们开发了一种含有 3 种浓度 AgNP 的 HSB。结果表明，HSB 成功地将 AgNP 容纳在基质中，并以可控的方式释放它们。通过紫外可见吸收光谱描述了 HSB 释放 AgNP 的动力学。通过透射电子显微镜（TEM）和动态光散射（DLS）测量评估释放的纳米粒子。此外，还使用洋葱根尖试验评估了细胞毒性和遗传毒性。结果表明，在所有研究浓度下，HSB-AgNP 均无细胞毒性。在 HSB-AgNP 的两个最高浓度下观察到遗传毒性作用，但在最低浓度下没有观察到。因此，在最低浓度下添加 AgNP 可以提高 HSB 的药理活性，而不会对植物细胞产生毒性作用。因此，本文中提出的木菠萝乳胶生物膜具有血管生成、抗炎和抗菌特性，可被视为用于伤口愈合的有潜力的新型生物材料。
