Reinardy H C, Chapman J, Bodnar A G
Molecular Discovery Laboratory, Bermuda Institute of Ocean Sciences, 17 Biological Station, St George's GE01, Bermuda
Molecular Discovery Laboratory, Bermuda Institute of Ocean Sciences, 17 Biological Station, St George's GE01, Bermuda.
Biol Lett. 2016 Feb;12(2):20151057. doi: 10.1098/rsbl.2015.1057.
Sea urchins are noted for the absence of neoplastic disease and represent a novel model to investigate cellular and systemic cancer protection mechanisms. Following intracoelomic injection of the DNA alkylating agent methyl methanesulfonate, DNA damage was detected in sea urchin cells and tissues (coelomocytes, muscle, oesophagus, ampullae and gonad) by the alkaline unwinding, fast micromethod. Gene expression analyses of the coelomocytes indicated upregulation of innate immune markers, including genes involved in NF-κB signalling. Results suggest that activation of the innate immune system following DNA damage may contribute to the naturally occurring resistance to neoplastic disease observed in sea urchins.
海胆因无肿瘤疾病而闻名，是研究细胞和全身癌症保护机制的新型模型。在向海胆体腔注射DNA烷化剂甲磺酸甲酯后，通过碱性解旋快速微量法在海胆细胞和组织（体腔细胞、肌肉、食道、壶腹和性腺）中检测到了DNA损伤。对体腔细胞的基因表达分析表明，包括参与NF-κB信号传导的基因在内的先天免疫标志物上调。结果表明，DNA损伤后先天免疫系统的激活可能有助于海胆对肿瘤疾病的天然抗性。
