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双酚A诱导的表观遗传模式失调：对雌性斑马鱼繁殖的影响。

BPA-Induced Deregulation Of Epigenetic Patterns: Effects On Female Zebrafish Reproduction.

作者信息

Santangeli Stefania, Maradonna Francesca, Gioacchini Giorgia, Cobellis Gilda, Piccinetti Chiara Carla, Dalla Valle Luisa, Carnevali Oliana

机构信息

Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.

INBB Consorzio Interuniversitario di Biosistemi e Biostrutture, 00136 Roma, Italy.

出版信息

Sci Rep. 2016 Feb 25;6:21982. doi: 10.1038/srep21982.

DOI:10.1038/srep21982
PMID:26911650
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4766405/
Abstract

Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation of epigenetic mechanisms. To determine its effects on female reproductive physiology and investigate whether changes in the expression levels of genes related to reproduction are caused by histone modifications, BPA concentrations consistent with environmental exposure were administered to zebrafish for three weeks. Effects on oocyte growth and maturation, autophagy and apoptosis processes, histone modifications, and DNA methylation were assessed by Real-Time PCR (qPCR), histology, and chromatin immunoprecipitation combined with qPCR analysis (ChIP-qPCR). The results showed that 5 μg/L BPA down-regulated oocyte maturation-promoting signals, likely through changes in the chromatin structure mediated by histone modifications, and promoted apoptosis in mature follicles. These data indicate that the negative effects of BPA on the female reproductive system may be due to its upstream ability to deregulate epigenetic mechanism.

摘要

双酚A（BPA）是全球最常见的内分泌干扰化合物之一。它可能通过诱导表观遗传机制失调来干扰脊椎动物的繁殖。为了确定其对雌性生殖生理的影响，并研究与生殖相关基因表达水平的变化是否由组蛋白修饰引起，将与环境暴露一致的双酚A浓度施用于斑马鱼三周。通过实时定量聚合酶链反应（qPCR）、组织学以及染色质免疫沉淀结合qPCR分析（ChIP-qPCR）评估其对卵母细胞生长和成熟、自噬和凋亡过程、组蛋白修饰以及DNA甲基化的影响。结果表明，5μg/L的双酚A可能通过组蛋白修饰介导的染色质结构变化下调卵母细胞成熟促进信号，并促进成熟卵泡的凋亡。这些数据表明，双酚A对雌性生殖系统的负面影响可能归因于其在表观遗传机制失调方面的上游作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/ff4261cfd00f/srep21982-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/85435becfe82/srep21982-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/f886b8159777/srep21982-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/968385755be4/srep21982-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/ff4261cfd00f/srep21982-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/85435becfe82/srep21982-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/f886b8159777/srep21982-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/968385755be4/srep21982-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6574/4766405/ff4261cfd00f/srep21982-f4.jpg

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