[用反向被动乳胶凝集试验检测大肠杆菌产生的不耐热肠毒素]
[Detection of heat-labile enterotoxin produced by E. coli with reversed passive latex agglutination].
作者信息
Wang F I, Chang T Y, Chang Y S
机构信息
National Laboratories of Foods and Drugs, Department of Health, Executive Yuan Taipei, Toiwan, R.O.C.
出版信息
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1989 May;22(2):145-9.
From June 1987 to March 1988, 103 E. coli strains were collected from non-foodborne samples, foodborne samples, FDA (USA) etc. and analyzed for heat-labile E. coli enterotoxin (LT) by reversed passive latex agglutination (RPLA). The results showed that 84 strains were LT-positive, 19 strains were LT-negative. Forty six strains of enteropathogenic E. coli isolated from foodborne samples showed that 34 strains were LT-positive, 12 strains were negative. Four enterotoxigenic E. coli strains from FDA (USA) used as positive controls, can produce heat-labile E. coli enterotoxin and give positive results.
1987年6月至1988年3月，从非食源性样本、食源性样本、美国食品药品监督管理局（FDA）等收集了103株大肠杆菌菌株，并通过反向被动乳胶凝集试验（RPLA）分析了不耐热大肠杆菌肠毒素（LT）。结果显示，84株为LT阳性，19株为LT阴性。从食源性样本中分离出的46株致病性大肠杆菌显示，34株为LT阳性，12株为阴性。用作阳性对照的来自美国FDA的4株产肠毒素大肠杆菌菌株可产生不耐热大肠杆菌肠毒素并给出阳性结果。
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