用于检测培养上清液中大肠杆菌不耐热毒素的反向被动乳胶凝集试验的评估
Evaluation of a reversed passive latex agglutination test for detection of Escherichia coli heat-labile toxin in culture supernatants.
作者信息
Scotland S M, Flomen R H, Rowe B
机构信息
Division of Enteric Pathogens, Central Public Health Laboratory, London, England.
出版信息
J Clin Microbiol. 1989 Feb;27(2):339-40. doi: 10.1128/jcm.27.2.339-340.1989.
Abstract
One hundred strains of Escherichia coli were tested for the production of the heat-labile enterotoxin by the Y1 adrenal cell test and a commercially available reversed passive latex agglutination test. The strains were grown in Casamino Acids-yeast extract broth, and filtered culture supernatants were tested for the presence of heat-labile enterotoxin. There was perfect correlation between the Y1 test and the reversed passive latex agglutination test, and the latter was simple to perform and completed within 48 h.
摘要
通过Y1肾上腺细胞试验和市售的反向被动乳胶凝集试验，对100株大肠杆菌进行了热不稳定肠毒素产生情况的检测。这些菌株在酪蛋白氨基酸 - 酵母提取物肉汤中培养，对过滤后的培养上清液进行热不稳定肠毒素检测。Y1试验和反向被动乳胶凝集试验结果完全相关，且后者操作简单，48小时内即可完成。
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