两种用于检测大肠杆菌不耐热肠毒素的商业凝集试剂盒的评估

Assessment of two commercial agglutination kits for detecting Escherichia coli heat-labile enterotoxin.

作者信息

Speirs J, Stavric S, Buchanan B

机构信息

Bureau of Microbial Hazards, Health and Welfare Canada, Ottawa, Ont.

出版信息

Can J Microbiol. 1991 Nov;37(11):877-80. doi: 10.1139/m91-151.

DOI:10.1139/m91-151

PMID:1777863

Abstract

Two commercial agglutination kits, a reserved passive agglutination test (VET-RPLA) and a staphylococcal coagglutination test (Phadebact ETEC-LT Test), were compared with two cell culture assays (Y-1 and Vero) and GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) for sensitivity in detecting Escherichia coli heat-labile enterotoxin (LT). Of 48 toxigenic strains, 23 were positive by all assays. One strain was negative only by the Phadebact test. Four strains, all LT-II producers, were positive by cell culture only. For LT-I detection, the Phadebact test was the least sensitive but was simple and rapid; VET-RPLA was simple, sensitive, and a good substitute for cell culture or GM1-ELISA.

摘要

将两种商业凝集试剂盒，即储备被动凝集试验（VET-RPLA）和葡萄球菌协同凝集试验（Phadebact ETEC-LT试验），与两种细胞培养测定法（Y-1和Vero）以及GM1神经节苷脂酶联免疫吸附测定法（GM1-ELISA）进行比较，以检测它们在检测大肠杆菌不耐热肠毒素（LT）方面的敏感性。在48株产毒菌株中，有23株在所有测定法中均呈阳性。有1株仅在Phadebact试验中呈阴性。4株均为LT-II产生菌，仅在细胞培养中呈阳性。对于LT-I的检测，Phadebact试验敏感性最低，但操作简单快速；VET-RPLA操作简单、敏感，是细胞培养或GM1-ELISA的良好替代方法。