用于检测大肠杆菌热不稳定肠毒素的Y1小鼠肾上腺细胞检测法与协同凝集试验的比较
Comparison of Y1 mouse adrenal cell and coagglutination assays for detection of Escherichia coli heat labile enterotoxin.
作者信息
Chapman P A, Daly C M
机构信息
Public Health Laboratory, Northern General Hospital, Sheffield.
出版信息
J Clin Pathol. 1989 Jul;42(7):755-8. doi: 10.1136/jcp.42.7.755.
Abstract
A commercial coagglutination assay (COA; Phadebact LT-ETEC) was compared with a Y1 mouse adrenal cell assay for detecting the heat labile enterotoxin of Escherichia coli. Of four different media evaluated for use with the COA, only one (modified blood agar) gave a positive result with all strains known to produce heat labile enterotoxin. With modified blood agar, the COA detected 74 (85%) of 87 such strains. Eighty six strains negative by cell culture assay were also negative by COA, and one strain positive by COA could not be confirmed by cell culture. The Phadebact LT-ETEC kit provides a simple, sensitive, and economical method for detecting E coli heat labile enterotoxin.
摘要
将一种商业性协同凝集试验（COA；Phadebact LT - ETEC）与Y1小鼠肾上腺细胞试验进行比较，以检测大肠杆菌的热不稳定肠毒素。在评估用于COA的四种不同培养基中，只有一种（改良血琼脂）对所有已知产生热不稳定肠毒素的菌株给出了阳性结果。使用改良血琼脂时，COA检测出87株此类菌株中的74株（85%）。细胞培养试验呈阴性的86株菌株经COA检测也为阴性，而COA检测呈阳性的1株菌株经细胞培养无法确认。Phadebact LT - ETEC试剂盒为检测大肠杆菌热不稳定肠毒素提供了一种简单、灵敏且经济的方法。
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