Department of Biophysics, Armed Forces Institute of Pathology, 810 Vermont Avenue, N.W., Washington, DC 20420, USA.
J Clin Microbiol. 2010 Dec;48(12):4620-2. doi: 10.1128/JCM.02019-10. Epub 2010 Oct 20.
Practical detection of cholera toxin (CT) by a liposome PCR (LPCR) immunoassay was compared to that of an established V. cholerae enterotoxin and Escherichia coli heat-labile enterotoxin reversed passive latex agglutination (VET-RPLA) assay. LPCR detected CT in the range of 10 pg/ml to 100 ng/ml in simulated feces and environmental water. Detection by VET-RPLA required at least 4 to 19 ng/ml CT.
通过脂质体 PCR(LPCR)免疫测定法对霍乱毒素(CT)进行实际检测,并与已建立的霍乱肠毒素和大肠杆菌不耐热肠毒素反向被动乳胶凝集(VET-RPLA)检测法进行比较。LPCR 在模拟粪便和环境水中检测到 CT 的范围为 10pg/ml 至 100ng/ml。VET-RPLA 的检测要求至少 4 至 19ng/ml CT。