Wang Y, Goodman M, Lumerman J, Sussman K E, Dahl R, Lafferty K J, Draznin B
Barbara Davis Center for Childhood Diabetes, Denver, CO.
Diabetes Res Clin Pract. 1989 Sep 18;7(3):205-11. doi: 10.1016/0168-8227(89)90006-5.
Recombinant interleukin-1 beta (IL-1 beta) was administered intraperitoneally for 3 days to normal C57BL/6ByJ (B6) mice. The islets from IL-1-treated and control animals were isolated and glucose-stimulated insulin secretion studied in the perifusion system. The total islet insulin content and the ultrastructure of the islets isolated from the animals treated with IL-1 did not differ from those seen in control animals. However, glucose-stimulated insulin release was significantly impaired after 3 days of in vivo administration of IL-1, either 3 micrograms/animal/day or 0.3 micrograms/animal/day. The administration of IL-1 inhibited an acute phase of glucose-induced insulin release, whereas neither basal insulin secretion nor insulin release from 10-30 min of perifusion with glucose was impaired. There was an only partial (27%) and non-significant restoration of the insulin secretory response to glucose stimulation 4 days after discontinuation of IL-1 treatment. We conclude that IL-1 administered in vivo is capable of adversely affecting pancreatic islet response to glucose stimulation. After 3 days of administration, these changes are confined to the process of insulin release, with the islet cell morphology and total insulin content being unaffected.
将重组白细胞介素-1β（IL-1β）腹腔注射给正常的C57BL/6ByJ（B6）小鼠，持续3天。分离经IL-1处理的动物和对照动物的胰岛，并在灌流系统中研究葡萄糖刺激的胰岛素分泌。从经IL-1处理的动物分离的胰岛的总胰岛素含量和超微结构与对照动物所见的无差异。然而，在体内给予IL-1 3天（3微克/动物/天或0.3微克/动物/天）后，葡萄糖刺激的胰岛素释放显著受损。IL-1的给药抑制了葡萄糖诱导的胰岛素释放的急性期，而基础胰岛素分泌和葡萄糖灌流10 - 30分钟时的胰岛素释放均未受损。在停止IL-1治疗4天后，对葡萄糖刺激的胰岛素分泌反应仅有部分（27%）且无显著恢复。我们得出结论，体内给予IL-1能够对胰岛对葡萄糖刺激的反应产生不利影响。给药3天后，这些变化仅限于胰岛素释放过程，胰岛细胞形态和总胰岛素含量未受影响。
