Zawalich W S, Diaz V A
Diabetes. 1986 Oct;35(10):1119-23. doi: 10.2337/diab.35.10.1119.
Preincubation of collagenase-isolated rat islets for 150 min with 100 U/ml purified human interleukin 1 (IL-1) altered their ability to secrete insulin. Whereas basal release rates with 4 mM glucose were comparable in control and IL-1-treated islets, both the first and second phases of release in response to 20 mM glucose were significantly reduced from IL-1-treated tissue. IL-1 pretreatment also impaired the secretory response to the combination of 100 nM cholecystokinin plus 7 mM glucose. However, the secretory response to 10 mM alpha-ketoisocaproate was comparable in control and IL-1-pretreated islets. Reducing the IL-1 exposure time to 60 min was accompanied by an augmented first phase of release to 20 mM glucose. Second phase secretion was diminished. The use of glucose measured after the perifusion was similar in control and IL-1-treated islets. Similar to other compounds that adversely impact on beta-cell viability, the inhibitory effect of IL-1 on release may presage a cytotoxic action of monokine.
用100 U/ml纯化的人白细胞介素1（IL-1）将胶原酶分离的大鼠胰岛预孵育150分钟，会改变它们分泌胰岛素的能力。虽然在对照胰岛和经IL-1处理的胰岛中，4 mM葡萄糖时的基础释放率相当，但经IL-1处理的组织对20 mM葡萄糖的释放的第一和第二阶段均显著降低。IL-1预处理也损害了对100 nM胆囊收缩素加7 mM葡萄糖组合的分泌反应。然而，对照胰岛和经IL-1预处理的胰岛对10 mMα-酮异己酸的分泌反应相当。将IL-1暴露时间缩短至60分钟，伴随着对20 mM葡萄糖释放的第一阶段增强。第二阶段分泌减少。对照胰岛和经IL-1处理的胰岛中灌注后测量的葡萄糖使用情况相似。与其他对β细胞活力有不利影响的化合物类似，IL-1对释放的抑制作用可能预示着单因子的细胞毒性作用。
