Hastie Kathryn M, Zandonatti Michelle, Liu Tong, Li Sheng, Woods Virgil L, Saphire Erica Ollmann
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA.
Department of Medicine, University of California-San Diego, La Jolla, California, USA.
J Virol. 2016 Apr 14;90(9):4556-62. doi: 10.1128/JVI.02896-15. Print 2016 May.
The arenavirus matrix protein Z is highly multifunctional and occurs in both monomeric and oligomeric forms. The crystal structure of a dodecamer of Z from Lassa virus, presented here, illustrates a ring-like structure with a highly basic center. Mutagenesis demonstrates that the dimeric interface within the dodecamer and a Lys-Trp-Lys triad at the center of the ring are important for oligomerization. This structure provides an additional template to explore the many functions of Z.
The arenavirus Lassa virus causes hundreds of thousands of infections each year, many of which develop into fatal hemorrhagic fever. The arenavirus matrix protein Z is multifunctional, with at least four distinct roles. Z exists in both monomeric and oligomeric forms, each of which likely serves a specific function in the viral life cycle. Here we present the dodecameric form of Lassa virus Z and demonstrate that Z forms a "wreath" with a highly basic center. This structure and that of monomeric Z now provide a pair of critical templates by which the multiple roles of Z in the viral life cycle may be interpreted.
沙粒病毒基质蛋白Z具有高度多功能性，以单体和寡聚体形式存在。本文展示的拉沙病毒Z的十二聚体晶体结构呈现出一个具有高度碱性中心的环状结构。诱变实验表明，十二聚体内的二聚体界面以及环中心的赖氨酸 - 色氨酸 - 赖氨酸三联体对寡聚化很重要。该结构为探索Z的多种功能提供了额外的模板。
沙粒病毒拉沙病毒每年导致数十万感染，其中许多发展为致命的出血热。沙粒病毒基质蛋白Z具有多种功能，至少有四个不同的作用。Z以单体和寡聚体形式存在，每种形式可能在病毒生命周期中发挥特定功能。在这里，我们展示了拉沙病毒Z的十二聚体形式，并证明Z形成了一个具有高度碱性中心的“花环”。这种结构和单体Z的结构现在提供了一对关键模板，通过它们可以解释Z在病毒生命周期中的多种作用。
