Song Yuqin, Sun Zhihong, Zhang Heping
Wei Sheng Wu Xue Bao. 2015 Nov 4;55(11):1371-7.
Lactic acid bacteria (LAB) are important organisms in the food industry. The study of microevolution of LAB is helpful in understanding of the biological function and mechanism of these microbes. With the development of molecular biology, a large number of technical means have emerged, such as multilocus sequence typing ( MLST) and whole-genome re-sequencing, which enable the study of the phylogenetic and population evolution of LAB at genetic level. MLST has already been widely used on microevolution research of LAB to analyze the genetic diversity and population structure. Moreover, recently, as a result of the declining in sequencing cost, the advantage of whole genome sequencing technology is increasingly highlighted. This article elucidates the principle, methods and scientific significance of researching LAB microevolution, as well as introduces the application of whole genome sequencing in these aspects to provide new insights into further research.
乳酸菌（LAB）是食品工业中的重要微生物。对乳酸菌微观进化的研究有助于理解这些微生物的生物学功能和机制。随着分子生物学的发展，出现了大量技术手段，如多位点序列分型（MLST）和全基因组重测序，这些技术能够在基因水平上研究乳酸菌的系统发育和群体进化。MLST已广泛应用于乳酸菌微观进化研究，以分析遗传多样性和群体结构。此外，近年来，由于测序成本下降，全基因组测序技术的优势日益凸显。本文阐述了研究乳酸菌微观进化的原理、方法和科学意义，并介绍了全基因组测序在这些方面的应用，为进一步研究提供新的见解。
