Chen Liang, Zhong Ruqing, Zhang Lilan, Zhang Hongfu
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Microorganisms. 2019 Mar 24;7(3):92. doi: 10.3390/microorganisms7030092.
The experiment was to determine the chronic effects of two transgenic maize lines that contained the gene from the strain (BT) and the gene from strain (CC), respectively, on ileal microbiota of laying hens. Seventy-two laying hens were randomly assigned to one of the three dietary treatments for 12 weeks, as follows: (1) nontransgenic near-isoline maize-based diet (CT diet), (2) BT maize-based diet (BT diet), and (3) CC maize-based diet (CC diet). Ileum histological examination did not indicate a chronic effect of two transgenic maize diets. Few differences were observed in any bacterial taxa among the treatments that used high-throughput 16S rRNA gene sequencing. The only differences that were observed for bacterial genera were that belong within the family tended to be greater ( = 0.114) abundant in hens fed the transgenic maize-based diet than in hens fed the CT diet. Birds that consumed the CC maize diet tended to have less abundance ( = 0.135) of family in the ileum than those that consumed the CT maize diet. These results indicate the lack of adverse effects of the BT maize and the CC maize lines on the ileal microbiota of hens for long term and provide important data regarding biosafety assessment of the transgenic maize lines.
该实验旨在确定分别含有来自苏云金芽孢杆菌菌株（BT）的基因和来自凝结芽孢杆菌菌株（CC）的基因的两种转基因玉米品系对蛋鸡回肠微生物群的慢性影响。72只蛋鸡被随机分配到三种日粮处理之一，为期12周，如下：（1）基于非转基因近等基因系玉米的日粮（CT日粮），（2）基于BT玉米的日粮（BT日粮），以及（3）基于CC玉米的日粮（CC日粮）。回肠组织学检查未显示两种转基因玉米日粮的慢性影响。在使用高通量16S rRNA基因测序的处理之间，在任何细菌分类群中观察到的差异很少。观察到的细菌属的唯一差异是，在以转基因玉米为基础日粮喂养的母鸡中，属于芽孢杆菌科的芽孢杆菌属往往比以CT日粮喂养的母鸡更为丰富（P = 0.114）。食用CC玉米日粮的鸡回肠中芽孢杆菌科的丰度往往低于食用CT玉米日粮的鸡（P = 0.135）。这些结果表明，BT玉米和CC玉米品系对母鸡回肠微生物群长期没有不良影响，并为转基因玉米品系的生物安全性评估提供了重要数据。
