State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
National Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China.
J Sci Food Agric. 2020 Dec;100(15):5450-5457. doi: 10.1002/jsfa.10596. Epub 2020 Jul 27.
The present study investigated the chronic effect on the composition and proportions of the cecal microbiota of laying hens for 12 weeks after consuming two genetically modified (GM) corns containing the maroACC gene from the Agrobacterium tumefaciens strain (CC) and the mCry1Ac gene from the Bacillus thuringiensis strain (BT) in comparison with the isogenic corn (CT).
In total, 72 hens were randomly assigned to the CT corn-based diet, CC corn-based diet and BT corn-based diet. The absolute weights of abdominal fat, breast, thigh meat and organ weight were not affected by the dietary treatment. High-throughput 16S rRNA gene sequencing revealed a few differences in the composition of cecal microbiota among the treatments. The only difference with respect to bacterial family was that the cecal abundance of Porphyromonadaceae (3.46 versus 2.11%; P = 0.073) tended to be higher for birds consuming the CC diet than those birds consuming the CT diet. Birds fed the BT diet tended to have a higher abundance of Barnesiella (0.62 versus 0.13%; P = 0.057) and a lower abundance of unclassified Ruminococcaceae (0.64 versus 1.19%; P = 0.097) than those fed the CT diet. Considering beneficial intestinal Barnesiella, this decreases and ultimately clears the colonization of vancomycin-resistant Enterococcus. The unclassified Ruminococcaceae was a low-frequency and low-abundance bacterial taxa and was not associated with intestinal pathology.
These results indicate a similar modulation of cecal microbiota in laying hens by long-term feeding among transgenic CC corn, BT corn and non-transgenic corn and provide data for biosafety evaluation of the transgenic corn. © 2020 Society of Chemical Industry.
本研究比较了食用含 maroACC 基因的农杆菌（CC）和含 mCry1Ac 基因的苏云金芽孢杆菌（BT）的两种转基因玉米以及与其同基因的玉米（CT） 12 周后对蛋鸡盲肠微生物组成和比例的慢性影响。
总共 72 只母鸡被随机分配到 CT 玉米基础日粮、CC 玉米基础日粮和 BT 玉米基础日粮。日粮处理对腹部脂肪、胸肌、大腿肉和器官重量的绝对重量没有影响。高通量 16S rRNA 基因测序显示，盲肠微生物组成在处理之间存在一些差异。唯一与细菌科有关的差异是，与食用 CT 日粮的鸡相比，食用 CC 日粮的鸡盲肠中卟啉单胞菌科的丰度（3.46 比 2.11%；P=0.073）较高。与食用 CT 日粮的鸡相比，食用 BT 日粮的鸡盲肠 Barnesiella 的丰度较高（0.62 比 0.13%；P=0.057），未分类的 Ruminococcaceae 的丰度较低（0.64 比 1.19%；P=0.097）。考虑到有益的肠道 Barnesiella，这会减少并最终清除万古霉素抗性肠球菌的定植。未分类的 Ruminococcaceae 是一种低频低丰度的细菌类群，与肠道病理无关。
这些结果表明，长期食用转基因 CC 玉米、BT 玉米和非转基因玉米对蛋鸡盲肠微生物群具有相似的调节作用，为转基因玉米的生物安全性评价提供了数据。 © 2020 化学工业协会。
