Miller C C, Samani N J, Carter A T, Brooks J I, Brammar W J
ICI/University Joint Laboratory, University of Leicester, UK.
J Hypertens. 1989 Nov;7(11):861-3. doi: 10.1097/00004872-198911000-00002.
We have studied the effect of dietary NaCl loading on renin gene expression in one-gene, two-gene and transgenic mouse strains. By Northern blotting, we found an approximate twofold reduction in renin messenger (m) RNA in the kidneys of high-NaCl-treated compared with low-NaCl-treated animals. Using an RNase-protection assay designed to discriminate between the different renin gene transcripts, we have shown that renin mRNAs derived from the Ren-1C gene of one-gene strains and the Ren-1D and Ren-2 genes of two-gene animals are all NaCl-responsive. Renin mRNA derived from a 19 kilobase Ren-1D transgene is also NaCl-responsive.
我们研究了饮食中氯化钠负荷对单基因、双基因和转基因小鼠品系肾素基因表达的影响。通过Northern印迹法，我们发现与低氯化钠处理的动物相比，高氯化钠处理的动物肾脏中肾素信使（m）RNA大约减少了两倍。使用一种旨在区分不同肾素基因转录本的核糖核酸酶保护分析方法，我们已经表明，来自单基因品系的Ren-1C基因以及双基因动物的Ren-1D和Ren-2基因的肾素mRNA均对氯化钠有反应。来自一个19千碱基Ren-1D转基因的肾素mRNA也对氯化钠有反应。
