猫乳腺癌中Ki-67和裂解型半胱天冬酶-3表达的组织学评估。
Histologic evaluation of Ki-67 and cleaved caspase-3 expression in feline mammary carcinoma.
作者信息
Silva Millena N, Leite Juliana S, Mello Marcela Fv, Silva Kassia Vgc, Corgozinho Katia B, de Souza Heloisa Jm, Cunha Simone Cs, Ferreira Ana Mr
机构信息
1 Postgraduate Program in Pathology, Fluminense Federal University, Niterói, RJ, Brazil.
2 Department of Pathology and Veterinary Clinics, Faculty of Veterinary Medicine, Fluminense Federal University Niteroi, RJ, Brazil.
出版信息
J Feline Med Surg. 2017 Apr;19(4):440-445. doi: 10.1177/1098612X16634150. Epub 2016 Jul 9.
Objectives The purpose of this study was to evaluate histopathologic aspects of, and the expression of Ki-67 and cleaved caspase-3 in, feline mammary carcinoma (FMC). Methods Feline mammary tumors were surgically obtained by mastectomy from 30 female cats and were fixed with formalin and embedded in paraffin wax. Four-micron sections were stained with hematoxylin and eosin for histopathologic diagnosis. Ki-67 and cleaved caspase-3 were analyzed by immunohistochemistry. Results Samples were histologically confirmed as FMC. Positive immunostaining was observed in all cancer samples for both nuclear Ki-67 and cleaved caspase-3, with a mean positive staining percentage of 27.5% and 21.2%, respectively. No statistically significant correlations between Ki-67 and cleaved caspase-3 were observed within FMC. Conclusions and relevance A high proliferation index was found in feline mammary tumors. This is the first study evaluating cleaved caspase-3 expression in FMC.
目的 本研究旨在评估猫乳腺癌（FMC）的组织病理学特征以及Ki-67和裂解型半胱天冬酶-3的表达情况。方法 通过乳房切除术从30只雌性猫身上手术获取猫乳腺肿瘤，用福尔马林固定并石蜡包埋。4微米切片用苏木精和伊红染色进行组织病理学诊断。通过免疫组织化学分析Ki-67和裂解型半胱天冬酶-3。结果 样本经组织学确认为FMC。在所有癌症样本中均观察到核Ki-67和裂解型半胱天冬酶-3的阳性免疫染色，平均阳性染色百分比分别为27.5%和21.2%。在FMC中未观察到Ki-67和裂解型半胱天冬酶-3之间有统计学意义的相关性。结论与意义 在猫乳腺肿瘤中发现了高增殖指数。这是第一项评估FMC中裂解型半胱天冬酶-3表达的研究。
Zotero 插件