Castagnaro M, Casalone C, Bozzetta E, De Maria R, Biolatti B, Caramelli M
Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria, Università degli Studi di Torino, Italy.
J Comp Pathol. 1998 Oct;119(3):263-75. doi: 10.1016/s0021-9975(98)80049-2.
To investigate whether the degree of differentiation in feline mammary carcinoma (FMC) can indicate the post-surgical survival time (PST), tumours were surgically resected from 55 cats and histologically graded according to a method derived from human breast cancer studies. One year after the resection, 26 cats (47.3%) were alive while 29 (52.7%) had died as a consequence of FMC. Formalin-fixed, paraffin wax-embedded sections stained with haematoxylin and eosin were used to classify the FMCs initially, according to the WHO system. Histological grading was then performed on the basis of three main features: degree of tubule formation, nuclear and cellular pleomorphism, and accurate mitotic count obtained from a defined area. Immunohistochemical examination with an anti-actin antibody was used for the accurate detection of "in situ" carcinomas. Age and histological type were not significantly correlated with the PST. Seven tumours (12.7%) were graded as well-differentiated carcinoma (WDC; grade I), 33 (60%) as moderately differentiated carcinoma (MDC; grade II), and 15 (27.3%) as poorly differentiated carcinoma (PDC; grade III). The tumour-related death rates after the first post-surgical year were 0 in cats with WDC, 14 (42.4%) in those with MDC, and 15 (100%) in those with PDC. Six cats with tumours showing extensive myoepithelial differentiation were all alive after 1 post-surgical year. The grading system seemed to have a good predictive value in respect of grades I and III of FMC but not for grade II. Myoepithelial differentiation may be relevant to clinical prognosis in FMC.
为了研究猫乳腺癌（FMC）的分化程度是否能表明术后生存时间（PST），对55只猫的肿瘤进行了手术切除，并根据源自人类乳腺癌研究的方法进行组织学分级。切除术后一年，26只猫（47.3%）存活，而29只（52.7%）因FMC死亡。最初，根据世界卫生组织系统，使用苏木精和伊红染色的福尔马林固定石蜡包埋切片对FMC进行分类。然后根据三个主要特征进行组织学分级：小管形成程度、核和细胞多形性以及从定义区域获得的准确有丝分裂计数。使用抗肌动蛋白抗体进行免疫组织化学检查以准确检测“原位”癌。年龄和组织学类型与PST无显著相关性。7个肿瘤（12.7%）被分级为高分化癌（WDC；I级），33个（60%）为中分化癌（MDC；II级），15个（27.3%）为低分化癌（PDC；III级）。术后第一年，WDC猫的肿瘤相关死亡率为0，MDC猫为14只（42.4%），PDC猫为15只（100%）。6只肿瘤显示广泛肌上皮分化的猫在术后1年全部存活。该分级系统对FMC的I级和III级似乎具有良好的预测价值，但对II级则不然。肌上皮分化可能与FMC的临床预后相关。
