嗜银性核仁组成区（AgNORs）计数作为猫乳腺癌术后预后的指标。

Argyrophilic nucleolar organiser regions (AgNORs) count as indicator of post-surgical prognosis in feline mammary carcinomas.

作者信息

Castagnaro M, Casalone C, Ru G, Nervi G C, Bozzetta E, Caramelli M

机构信息

Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria, Università degli Studi di Torino, Italy.

出版信息

Res Vet Sci. 1998 Mar-Apr;64(2):97-100. doi: 10.1016/s0034-5288(98)90002-9.

DOI:10.1016/s0034-5288(98)90002-9

PMID:9625463

Abstract

Feline mammary carcinomas (FMC) were surgically resected from 51 cats to verify that the nucleolar organiser regions (AgNORs) count is related to the post-surgical survival time (PST). After one year post-surgery, 27 cats (group A) were still alive (52.9 per cent) while (41.7 per cent) (group B) had died as a consequence of FMC. Formalin-fixed, paraffin wax-embedded histological sections were stained with silver nitrate and the AgNORs were then counted at x 100 oil immersion objective. In FMC AgNORs count ranged from 1.2 to 12.1 (6.10+/-2.3). A statistically significant difference (P=0.000112) in the AgNORs count was found between cats from group A and group B. No other statistically significant differences were found between group A and B. AgNORs count was not correlated to age or different histological type, according to the WHO classification. Using this technique it is possible to identify two populations of FMC with a statistically significant difference in the PST.

摘要

从51只猫身上手术切除猫乳腺肿瘤（FMC），以验证核仁组成区（AgNORs）计数与术后存活时间（PST）是否相关。术后一年，27只猫（A组）仍存活（52.9%），而（41.7%）（B组）因FMC死亡。用硝酸银对福尔马林固定、石蜡包埋的组织学切片进行染色，然后在油镜×100下对AgNORs进行计数。在FMC中，AgNORs计数范围为1.2至12.1（6.10±2.3）。在A组和B组的猫之间，AgNORs计数存在统计学显著差异（P = 0.000112）。在A组和B组之间未发现其他统计学显著差异。根据世界卫生组织的分类，AgNORs计数与年龄或不同组织学类型无关。使用该技术可以识别出两个FMC群体，它们在PST方面存在统计学显著差异。