Lin Guohe, Liu Yongcheng, Li Shuhong, Mao Yize, Wang Jun, Shuang Zeyu, Chen Jianlin, Li Shengping
State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangzhou, China.
National Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.
Oncotarget. 2016 Aug 9;7(32):50963-50971. doi: 10.18632/oncotarget.7680.
We investigated whether elevated neutrophil-to-lymphocyte ratio ( NLR ) was associated with poor anti-tumor immunity and prognosis in patients with intrahepatic cholangiocarcinoma ( ICC ). Clinicopathologic data of 102 patients with ICC who underwent hepatectomy was retrospectively analyzed. The Kaplan-Meier method and Cox regression model were used to analyze the survival and prognosis. The percentage of overall lymphocytes , T cells and CD8+ T cells in the high NLR group was lower than that in the low NLR group. The percentage of PD-1+CD4+ and PD-1+CD8+ T cells was higher and the percentage of IFN-γ+CD4+ and IFN-γ+CD8+ T cells was lower in the high NLR group than that in the low NLR group ( p = 0.045, p = 0.008; p = 0.012, p = 0.006 ). Density of tumor-infiltrating CD3+ T cells in the high NLR group was lower than that in the low NLR group ( p < 0.001 ). Elevated NLR was an independent predictor for poor overall survival ( OS; p = 0.035 ) and recurrence-free survival ( RFS; p = 0.008 ). These results indicate that elevated NLR is associated with poor anti-tumor immunity and could be a poor biomarker for prognosis in patients with ICC.
我们研究了中性粒细胞与淋巴细胞比值（NLR）升高是否与肝内胆管癌（ICC）患者抗肿瘤免疫功能差及预后不良相关。回顾性分析了102例行肝切除术的ICC患者的临床病理资料。采用Kaplan-Meier法和Cox回归模型分析生存情况和预后。高NLR组中总淋巴细胞、T细胞和CD8+T细胞的百分比低于低NLR组。高NLR组中PD-1+CD4+和PD-1+CD8+T细胞的百分比高于低NLR组，而IFN-γ+CD4+和IFN-γ+CD8+T细胞的百分比低于低NLR组（p = 0.045，p = 0.008；p = 0.012，p = 0.006）。高NLR组肿瘤浸润CD3+T细胞的密度低于低NLR组（p < 0.001）。NLR升高是总生存期（OS；p = 0.035）和无复发生存期（RFS；p = 0.008）不良的独立预测因素。这些结果表明，NLR升高与抗肿瘤免疫功能差相关，可能是ICC患者预后不良的生物标志物。
