Mohanta Tapan Kumar, Mohanta Nibedita, Parida Pratap, Panda Sujogya Kumar, Ponpandian Lakshmi Narayanan, Bae Hanhong
Free Major of Natural Science, College of Basic Studies, Yeungnam University, Gyeongsan, Gyeongsangbuk-do, 712749, Republic of Korea.
Department Of Biotechnology, North Orissa University, Takatpur, Baripada, 757003, India.
PLoS One. 2016 Feb 26;11(2):e0149861. doi: 10.1371/journal.pone.0149861. eCollection 2016.
The mitogen-activated protein kinase (MAPK) is characterized by the presence of the T-E-Y, T-D-Y, and T-G-Y motifs in its activation loop region and plays a significant role in regulating diverse cellular responses in eukaryotic organisms. Availability of large-scale genome data in the fungal kingdom encouraged us to identify and analyse the fungal MAPK gene family consisting of 173 fungal species. The analysis of the MAPK gene family resulted in the discovery of several novel activation loop motifs (T-T-Y, T-I-Y, T-N-Y, T-H-Y, T-S-Y, K-G-Y, T-Q-Y, S-E-Y and S-D-Y) in fungal MAPKs. The phylogenetic analysis suggests that fungal MAPKs are non-polymorphic, had evolved from their common ancestors around 1500 million years ago, and are distantly related to plant MAPKs. We are the first to report the presence of nine novel activation loop motifs in fungal MAPKs. The specificity of the activation loop motif plays a significant role in controlling different growth and stress related pathways in fungi. Hence, the presences of these nine novel activation loop motifs in fungi are of special interest.
丝裂原活化蛋白激酶（MAPK）的特征是在其激活环区域存在T-E-Y、T-D-Y和T-G-Y基序，并且在调节真核生物中多种细胞反应方面发挥着重要作用。真菌界大规模基因组数据的可得性促使我们鉴定和分析由173种真菌物种组成的真菌MAPK基因家族。对MAPK基因家族的分析导致在真菌MAPK中发现了几种新的激活环基序（T-T-Y、T-I-Y、T-N-Y、T-H-Y、T-S-Y、K-G-Y、T-Q-Y、S-E-Y和S-D-Y）。系统发育分析表明，真菌MAPK是非多态性的，大约在15亿年前从它们的共同祖先进化而来，并且与植物MAPK关系较远。我们是第一个报道真菌MAPK中存在九个新激活环基序的。激活环基序的特异性在控制真菌中不同的生长和应激相关途径方面起着重要作用。因此，真菌中这九个新激活环基序的存在具有特殊意义。
