Lilliehöök Inger, Tvedten Harold W, Bröjer Johan, Edner Anna, Nostell Katarina
University Animal Hospital, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Vet Clin Pathol. 2016 Mar;45(1):66-72. doi: 10.1111/vcp.12334. Epub 2016 Feb 26.
Neutrophil myeloperoxidase content is determined by the Advia 2120 hematology system by staining characteristics. Changes in myeloperoxidase staining are shown by location of neutrophils on Advia peroxidase dot plots and as myeloperoxidase index (MPXI). Significant changes in MPXI have been reported during severe inflammation in horses, dogs, and people but conclusions were inconsistent.
Infusion of endotoxin was used to initiate an inflammatory stimulus under controlled conditions and over a longer time period than in previous studies to document kinetics of changes in neutrophil numbers, morphology, and myeloperoxidase staining. Identification of consistent time-related changes may allow better interpretation of changes in neutrophil characteristics during inflammation.
Five Standardbred trotting horses received an intravenous infusion over a 6-hour period with Escherichia coli endotoxin. Neutrophil count, MPXI, neutrophil characteristics in Advia 2120 Perox dot plots and neutrophil morphology in blood smears were monitored with repeated sampling for up to 10 days.
Endotoxin infusion immediately caused severe neutropenia which converted to neutrophilia 14 hours after start of endotoxin infusion. Neutrophilia was still present 78 hours after start of infusion. Large "giant" neutrophils first appeared in blood smears and Advia Perox dot plots after 36-48 hours. A marked and consistent decrease in MPXI was seen in all horses 6 days (150 hours) after endotoxin exposure.
Endotoxemia caused prominent, time-related changes in equine neutrophil characteristics including emergence of giant neutrophils and markedly decreased MPXI several days after endotoxin infusion.
Advia 2120血液学系统通过染色特征来测定中性粒细胞髓过氧化物酶含量。中性粒细胞过氧化物酶染色的变化通过中性粒细胞在Advia过氧化物酶散点图上的位置以及髓过氧化物酶指数（MPXI）来显示。据报道，在马、狗和人类的严重炎症期间，MPXI有显著变化，但结论并不一致。
在内毒素输注过程中，与之前的研究相比，采用更长的时间并在可控条件下引发炎症刺激，以记录中性粒细胞数量、形态和髓过氧化物酶染色变化的动力学。识别与时间相关的一致变化可能有助于更好地解释炎症期间中性粒细胞特征的变化。
五匹标准赛马在6小时内静脉输注大肠杆菌内毒素。通过重复采样监测中性粒细胞计数、MPXI、Advia 2120过氧化物散点图中的中性粒细胞特征以及血涂片中性粒细胞形态，最长监测10天。
内毒素输注立即导致严重的中性粒细胞减少，在内毒素输注开始14小时后转变为中性粒细胞增多。输注开始78小时后仍存在中性粒细胞增多。36 - 48小时后，大的“巨型”中性粒细胞首次出现在血涂片和Advia过氧化物散点图中。在内毒素暴露6天（150小时）后，所有马匹的MPXI均出现显著且一致的下降。
内毒素血症导致马的中性粒细胞特征出现显著的、与时间相关的变化，包括巨型中性粒细胞的出现以及内毒素输注几天后MPXI明显下降。
