Wöhrer Daniela, Spergser Joachim, Bagrinovschi Gabriela, Möstl Karin, Weissenböck Herbert
Institute of Pathology and Forensic Veterinary Medicine, University of Veterinary Medicine Vienna , Veterinärplatz 1, A-1210 Vienna , Austria.
Institute of Microbiology, University of Veterinary Medicine Vienna , Veterinärplatz 1, A-1210 Vienna , Austria.
Acta Vet Hung. 2016 Mar;64(1):103-15. doi: 10.1556/004.2016.011.
The aim of this retrospective study was to detect selected pathogens in pneumonic lung tissue of dogs of different age groups by immunohistochemistry (IHC), in situ hybridisation (ISH) or polymerase chain reaction (PCR) in order to get information about their involvement in pneumonia formation. In archived formalin-fixed and paraffin wax-embedded lung samples from 68 cases with the clinical and histologic diagnosis of pneumonia the histological pattern of pneumonia was re-evaluated and the samples were further investigated for the following infectious agents: canine distemper virus (CDV), canine adenovirus type 2 (CAV-2), canine respiratory coronavirus (CRCoV), Bordetella (B.) bronchiseptica, Pasteurella (P.) multocida, Mycoplasma spp., and Pneumocystis spp. In 47.1% of the samples at least one of the featured respiratory pathogens was detected. In 31.3% of these positive samples more than one pathogen could be found. The correct detection of CDV had been achieved in ten out of eleven positive cases (90.9%) upon initial investigation, but the presence of bacterial pathogens, like B. bronchiseptica (10 cases) and P. multocida (17 cases) had been missed in all but one case. While CDV and CRCoV infections were exclusively found in dogs younger than one year, the vast majority of infections with P. multocida and B. bronchiseptica were both common either in dogs younger than 4 months or older than one year. Thus, this retrospective approach yielded valuable data on the presence, absence and prevalence of certain respiratory pathogens in dogs with pneumonia.
本回顾性研究的目的是通过免疫组织化学（IHC）、原位杂交（ISH）或聚合酶链反应（PCR）检测不同年龄组犬肺炎肺组织中的特定病原体，以获取它们在肺炎形成中所起作用的信息。在68例临床和组织学诊断为肺炎的存档福尔马林固定石蜡包埋肺样本中，重新评估肺炎的组织学模式，并对样本进一步检测以下感染因子：犬瘟热病毒（CDV）、犬腺病毒2型（CAV-2）、犬呼吸道冠状病毒（CRCoV）、支气管败血博德特氏菌（B. bronchiseptica）、多杀巴斯德氏菌（P. multocida）、支原体属和肺孢子菌属。在47.1%的样本中检测到至少一种上述呼吸道病原体。在这些阳性样本中，31.3%可发现不止一种病原体。初步调查时，11例阳性病例中有10例（90.9%）正确检测到CDV，但除1例之外，所有病例均未检测到细菌病原体，如支气管败血博德特氏菌（10例）和多杀巴斯德氏菌（17例）。虽然CDV和CRCoV感染仅在1岁以下犬中发现，但多杀巴斯德氏菌和支气管败血博德特氏菌感染绝大多数在4月龄以下或1岁以上犬中常见。因此，这种回顾性方法得出了关于肺炎犬中某些呼吸道病原体存在、不存在和流行情况的有价值数据。
