Weissenbacher-Lang Christiane, Blasi Barbara, Bauer Patricia, Binanti Diana, Bittermann Karin, Ergin Lara, Högler Carmen, Högler Tanja, Klier Magdalena, Matt Julia, Nedorost Nora, Silvestri Serenella, Stixenberger Daniela, Ma Liang, Cissé Ousmane H, Kovacs Joseph A, Desvars-Larrive Amélie, Posautz Annika, Weissenböck Herbert
Department for Pathobiology, Institute of Pathology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
Critical Care Medicine Department, NIH Clinical Center, National Institutes of Health (NIH), 10 Center Drive, Bethesda, MD 20892, USA.
J Fungi (Basel). 2023 Feb 7;9(2):220. doi: 10.3390/jof9020220.
spp. are thought to adapt to the lungs of potentially all mammals. However, the full host range, fungal burden and severity of infection are unknown for many species. In this study, lung tissue samples originating from 845 animals of 31 different families of eight mammal orders were screened by in situ hybridization (ISH) using a universal 18S rRNA probe for , followed by hematoxylin and eosin (H&E) staining for determining histopathological lesions. A total of 216 (26%) samples were positive for spp., encompassing 36 of 98 investigated mammal species, with 17 of them being described for the first time for the presence of spp. The prevalence of spp. as assessed by ISH varied greatly among different mammal species while the organism load was overall low, suggesting a status of colonization or subclinical infection. Severe pneumonia seemed to be very rare. For most of the -positive samples, comparative microscopic examination of H&E- and ISH-stained serial sections revealed an association of the fungus with minor lesions, consistent with an interstitial pneumonia. Colonization or subclinical infection of in the lung might be important in many mammal species because the animals may serve as a reservoir.
人们认为[物种名称]能够适应可能所有哺乳动物的肺部。然而，许多物种的完整宿主范围、真菌负荷和感染严重程度尚不清楚。在本研究中，使用针对[物种名称]的通用18S rRNA探针，通过原位杂交（ISH）对来自八个哺乳动物目31个不同科的845只动物的肺组织样本进行筛查，随后进行苏木精和伊红（H&E）染色以确定组织病理学病变。共有216份（26%）样本对[物种名称]呈阳性，涵盖98种被调查哺乳动物中的36种，其中17种是首次被描述存在[物种名称]。通过ISH评估的[物种名称]患病率在不同哺乳动物物种之间差异很大，而生物体负荷总体较低，表明处于定植或亚临床感染状态。严重的[物种名称]肺炎似乎非常罕见。对于大多数[物种名称]阳性样本，对H&E和ISH染色的连续切片进行比较显微镜检查发现，真菌与轻微病变有关，符合间质性肺炎。[物种名称]在肺部的定植或亚临床感染在许多哺乳动物物种中可能很重要，因为这些动物可能是储存宿主。
