Yan Shuai, Lai Guiyan, Li Li, Xiao Hao, Zhao Ming, Wang Ming
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
Nansha Entry-Exit Inspection and Quarantine Bureau, Guangzhou 511457, PR China.
Food Chem. 2016 Jul 1;202:116-9. doi: 10.1016/j.foodchem.2016.01.133. Epub 2016 Feb 1.
In the present study, we employed a DNA barcoding approach to authenticate the species of fish imported via one port in China. The fish were identified as smallmouth scad based on morphological characteristics, Alepes apercna (Perciformes, Carangidae), but were labeled as Rastrelliger brachysoma (Perciformes, Scombridae). Fragments of the partial mitochondrial cytochrome c oxidase 1 (COI) gene were sequenced from 12 specimens, and their phylogenetic relationship was subsequently examined. The phylogenetic analysis demonstrated that all of the individuals formed a monophyletic cluster with high bootstrap values and were placed in a sister group with the ancestor of Alepes vari and Alepes melanoptera. The K2P genetic distances at an intraspecific level were significantly smaller than those at an interspecific level. Our results indicated that the fish were A. apercna, rather than R. brachysoma, which confirms the morphological analysis. This study presents a practical demonstration of the use of DNA barcoding to prevent fraud in international trade.
在本研究中，我们采用DNA条形码技术对通过中国某一港口进口的鱼类品种进行鉴定。这些鱼根据形态特征被鉴定为小口鲹，即长体圆鲹（鲈形目，鲹科），但标签上标注为圆腹鲹（鲈形目，鲭科）。从12个样本中对部分线粒体细胞色素c氧化酶1（COI）基因片段进行了测序，并随后检测了它们的系统发育关系。系统发育分析表明，所有个体形成了一个具有高自展值的单系类群，并与长鳍圆鲹和黑鳍圆鲹的祖先处于姐妹群中。种内水平的K2P遗传距离显著小于种间水平的遗传距离。我们的结果表明，这些鱼是长体圆鲹，而不是圆腹鲹，这证实了形态学分析。本研究展示了DNA条形码技术在防止国际贸易欺诈方面的实际应用。
