Meng Xianhui, Sun Bo, Xue Mengying, Xu Peng, Hu Feihu, Xiao Zhongdang
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China.
Genomics. 2016 Apr;107(4):124-31. doi: 10.1016/j.ygeno.2016.02.006. Epub 2016 Feb 26.
Human mesenchymal stem cells (MSCs) derived from both umbilical cord (UC) and cord blood (CB) share similar characteristics, and their differences are largely unknown. Besides the significant difference in cell morphology, differentiation ability and development processes of the two different origin MSCs, a different expression pattern of microRNAs between the two kinds of MSCs was also obtained. By comprehensively annotating the differently expressed global microRNAs (miRNAs), a series of biological pathways were predicted. We found that miRNAs significantly repressed insulin signaling in UCMSCs, while neural related processes were more repressed in CBMSCs. Particularly, TGF-β and Notch signaling were differently activated in both MSCs, unveiling their distinct angiogenesis potentials. Taken together, this study illustrates that MSCs from UC and CB display distinct properties, which indicates different potentials for clinical usage.
源自脐带（UC）和脐带血（CB）的人间充质干细胞（MSC）具有相似的特征，但其差异在很大程度上尚不清楚。除了两种不同来源的MSC在细胞形态、分化能力和发育过程上存在显著差异外，两种MSC之间的微小RNA（miRNA）表达模式也有所不同。通过对差异表达的整体miRNA进行全面注释，预测了一系列生物学途径。我们发现，miRNA在脐带间充质干细胞（UCMSC）中显著抑制胰岛素信号通路，而在脐血间充质干细胞（CBMSC）中与神经相关的过程受到更多抑制。特别是，转化生长因子-β（TGF-β）和Notch信号通路在两种MSC中被不同程度地激活，揭示了它们不同的血管生成潜力。综上所述，本研究表明来自脐带和脐带血的MSC具有不同的特性，这表明它们在临床应用中具有不同的潜力。
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