用于解决生物学问题的化学成像方法概述。

Overview of chemical imaging methods to address biological questions.

作者信息

da Cunha Marcel Menezes Lyra, Trepout Sylvain, Messaoudi Cédric, Wu Ting-Di, Ortega Richard, Guerquin-Kern Jean-Luc, Marco Sergio

机构信息

Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Laboratório de Biologia Celular de Fungos, RJ 21044-020, Brazil.

INSERM, U1196, Orsay F-91405, France; Institut Curie, Centre de Recherche, Orsay F-91405, France; CNRS, UMR9187, Orsay F-91405, France; Université Paris-Sud, Orsay F-91405, France.

出版信息

Micron. 2016 May;84:23-36. doi: 10.1016/j.micron.2016.02.005. Epub 2016 Feb 11.

DOI:10.1016/j.micron.2016.02.005

PMID:26922256

Abstract

Chemical imaging offers extensive possibilities for better understanding of biological systems by allowing the identification of chemical components at the tissue, cellular, and subcellular levels. In this review, we introduce modern methods for chemical imaging that can be applied to biological samples. This work is mainly addressed to the biological sciences community and includes the bases of different technologies, some examples of its application, as well as an introduction to approaches on combining multimodal data.

摘要

化学成像通过能够在组织、细胞和亚细胞水平上识别化学成分，为更好地理解生物系统提供了广泛的可能性。在本综述中，我们介绍了可应用于生物样品的化学成像现代方法。这项工作主要面向生物科学界，内容包括不同技术的基础、其应用的一些实例，以及多模态数据结合方法的介绍。