Nguyen Sam Thi, Fukuda Yasuhiro, Nguyen Duc Tan, Tada Chika, Nakai Yutaka
Department of Parasitology, Central Vietnam Veterinary Institute, km4 Dongde street, Nhatrang, Vietnam.
Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, 232-3 Yomogida, Naruko-Onsen, Osaki, Miyagi, 989-6711, Japan.
Trop Anim Health Prod. 2016 Apr;48(4):837-41. doi: 10.1007/s11250-016-1013-x. Epub 2016 Feb 27.
Little information is available on the epidemiology of Giardia duodenalis in beef cattle from Vietnam. This study was performed to determine the prevalence and genotypes/assemblages of G. duodenalis in native beef calves younger than 6 months in the region. A total of 412 calf fecal samples, randomly selected from 99 small-scale farms located in DacLac and KhanhHoa provinces, central Vietnam, were screened for the presence of G. duodenalis cysts using the zinc-sulfate flotation method followed by iodine staining. The overall prevalence on the sample and herd levels were 13.8% (57/412) and 42.4% (42/99), respectively. Molecular analysis in the β-giardin and triosephosphate isomerase genes demonstrated the presence of only G. duodenalis assemblage E in the animals. Since assemblage E has been rarely reported in humans, the zoonotic risk in beef calves in the region appears to be minimal.
关于越南肉牛中十二指肠贾第虫的流行病学信息很少。本研究旨在确定该地区6个月龄以下本地肉牛犊中十二指肠贾第虫的流行率和基因型/组合。从越南中部多乐省和庆和省的99个小型农场随机选取了412份犊牛粪便样本，先用硫酸锌浮选法，再用碘染色法筛查十二指肠贾第虫囊肿的存在。样本水平和畜群水平的总体流行率分别为13.8%（57/412）和42.4%（42/99）。对β-贾第蛋白和磷酸丙糖异构酶基因的分子分析表明，动物中仅存在十二指肠贾第虫组合E。由于组合E在人类中很少被报道，该地区肉牛犊的人畜共患病风险似乎很小。
