Geng Xiwen, Xie Jinlu, Wang Xuenan, Wang Xiusong, Zhang Xiao, Hou Yabing, Lei Chengdong, Li Min, Qu Qingyang, He Tingting, Han Hongyu, Yao Xiaomeng, Wang Min
Key Laboratory of Animal Resistance of Shandong Province, College of Life Science, Shandong Normal University, Jinan 250014, People's Republic of China.
Key Laboratory of Animal Resistance of Shandong Province, College of Life Science, Shandong Normal University, Jinan 250014, People's Republic of China; Qinhuangdao Medical School, Qinhuangdao 066000, People's Republic of China.
Behav Brain Res. 2016 May 15;305:57-64. doi: 10.1016/j.bbr.2016.02.026. Epub 2016 Feb 26.
The pedunculopontine nucleus (PPN) is a new deep brain stimulation target for treating Parkinson's disease (PD). But the alterations of the PPN electrophysiological activities in PD are still debated. To investigate these potential alterations, extracellular single unit and local field potential (LFP) activities in the PPN were recorded in unilateral hemispheric 6-hydroxydopamine (6-OHDA) lesioned rats and in control rats, respectively. The spike activity results revealed two types of neurons (Type I and Type II) with distinct electrophysiological characteristics in the PPN. Both types of neurons had increased firing rate and changed firing pattern in lesioned rats when compared to control rats. Specifically, Type II neurons showed an increased firing rate when the rat state was switched from rest to locomotion. The LFP results demonstrated that lesioned rats had lower LFP power at 0.7-12Hz and higher power at 12-30Hz than did control animals in either resting or locomotor state. These findings provide a better understanding of the effects of 6-OHDA lesion on neuronal activities in the PPN and also provide a proof of the link between this structure and locomotion, which contributes to better understanding the mechanisms of the PPN functioning in the pathophysiology of PD.
脚桥核（PPN）是治疗帕金森病（PD）的一个新的脑深部刺激靶点。但PD中PPN电生理活动的改变仍存在争议。为了研究这些潜在的改变，分别在单侧半球6-羟基多巴胺（6-OHDA）损伤大鼠和对照大鼠中记录了PPN的细胞外单单位和局部场电位（LFP）活动。锋电位活动结果显示，PPN中有两种具有不同电生理特征的神经元（I型和II型）。与对照大鼠相比，两种类型的神经元在损伤大鼠中均有放电频率增加和放电模式改变。具体而言，当大鼠状态从静止转变为运动时，II型神经元的放电频率增加。LFP结果表明，在静息或运动状态下，损伤大鼠在0.7-12Hz时的LFP功率低于对照动物，而在12-30Hz时的功率高于对照动物。这些发现有助于更好地理解6-OHDA损伤对PPN神经元活动的影响，也为该结构与运动之间的联系提供了证据，有助于更好地理解PPN在PD病理生理学中的功能机制。
