Experimental Centre, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, Shandong, China.
Key Laboratory of Vector Biology and Pathogen Control of Zhejiang, School of Medicine, Huzhou University, Huzhou Central Hospital, Huzhou 313000, Zhejiang, China.
Aging (Albany NY). 2021 Feb 26;13(5):7314-7329. doi: 10.18632/aging.202585.
The pedunculopontine nucleus (PPN) undergoes significant anatomic and electrophysiological alterations in Parkinson's disease (PD), severely impacting locomotion. However, the effect of 6-hydroxydopamine (6-OHDA) lesion and levodopa (L-DOPA) therapy on the relationships between spike activities and local field potential (LFP) within the PPN is not well-understood. Synchronisation between the spike activity of individual neurones and LFP of neuronal ensembles is a crucial problem in the pathogenesis of PD. In this study, LFP signals and spikes in the PPN of rats in control, lesioned, and L-DOPA groups were recorded synchronously with a multi-unit electrical signal acquisition system and analysed for their coherence value, spike-field coherence, and phase-lock relationship. The spike-LFP relationship in the PPN was markedly increased in specific frequency bands because of the 6-OHDA lesion but differed depending on the animal locomotion state and neuronal type. L-DOPA had a limited therapeutic effect on the 6-OHDA-induced increase in the coherence value. Our study demonstrates that the PPN spike-LFP relationship is involved in the pathogenesis of PD and is critical for the effects of L-DOPA, providing a basis for the clinical treatment of refractory PD symptoms.
被盖部脑桥核（PPN）在帕金森病（PD）中经历显著的解剖和电生理改变，严重影响运动。然而，6-羟多巴胺（6-OHDA）损伤和左旋多巴（L-DOPA）治疗对 PPN 内尖峰活动和局部场电位（LFP）之间关系的影响尚不清楚。个体神经元尖峰活动和神经元集合 LFP 之间的同步是 PD 发病机制中的一个关键问题。在这项研究中，使用多单元电信号采集系统同步记录了对照组、损伤组和 L-DOPA 组大鼠 PPN 中的 LFP 信号和尖峰，并对其相干值、尖峰-场相干性和锁相关系进行了分析。由于 6-OHDA 损伤，PPN 中的尖峰-LFP 关系在特定频率带中显著增加，但因动物运动状态和神经元类型而异。L-DOPA 对 6-OHDA 诱导的相干值增加的治疗效果有限。我们的研究表明，PPN 的尖峰-LFP 关系参与了 PD 的发病机制，对 L-DOPA 的作用至关重要，为治疗难治性 PD 症状提供了临床依据。
