八只猫的骨髓中细小病毒的鉴定
Identification of parvovirus in the bone marrow of eight cats.
作者信息
Haynes S M, Holloway S A
机构信息
Small Animal Medicine Department, University of Melbourne, 250 Princes Hwy, Werribee, Victoria 3030, Australia.
出版信息
Aust Vet J. 2012 Apr;90(4):136-9. doi: 10.1111/j.1751-0813.2012.00899.x.
OBJECTIVE
To determine if canine parvovirus (CPV) or feline panleucopenia virus (FPV) genomic sequences are present in adult feline bone marrow samples.
DESIGN
Bone marrow samples were obtained from 32 semi-feral cats that were euthanased at an animal shelter. DNA was extracted and subjected to conventional polymerase chain reaction (PCR) designed to determine if CPV or FPV DNA was present. Positive PCR products were purified, cloned and sequenced to differentiate between CPV and FPV.
RESULTS
Eight of the bone marrow samples contained parvoviral DNA (7 CPV, 1 FPV).
CONCLUSION
CPV and FPV DNA can be found in the bone marrow of healthy adult cats.
目的
确定成年猫骨髓样本中是否存在犬细小病毒（CPV）或猫泛白细胞减少症病毒（FPV）的基因组序列。
设计
从一家动物收容所安乐死的32只半野生猫身上获取骨髓样本。提取DNA并进行常规聚合酶链反应（PCR），以确定是否存在CPV或FPV DNA。对阳性PCR产物进行纯化、克隆和测序，以区分CPV和FPV。
结果
8份骨髓样本含有细小病毒DNA（7份CPV，1份FPV）。
结论
在健康成年猫的骨髓中可发现CPV和FPV DNA。
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