未临产的胎膜早破与基质金属蛋白酶-9蛋白水平升高无关。

Preterm premature rupture of membranes without labor is not associated with increased levels of matrix metalloproteinase-9 protein.

作者信息

Draper D L, Kush M J, Donohoe W, Janosky J, Latimer J J, Heine R P

机构信息

Magee-Women's Research Institute, Department of Obstetrics and Gynecology and Reproductive Sciences, School of Medicine, University of Pittsburgh, Pennsylvania, USA.

Magee-Women's Research Institute, Department of Family Medicine and Clinical Epidemiology, School of Medicine, University of Pittsburgh, Pennsylvania, USA.

出版信息

Prenat Neonatal Med. 2001 Aug 1;6(4):219-226.

PMID:26924938

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4769030/

Abstract

OBJECTIVES

Preterm premature rupture of membranes (PROM) accounts for 30-40% of all preterm births. The objectives of this study were to determine whether matrix metalloproteinase-9 (MMP-9) is increased in preterm PROM fetal membranes, whether labor or gestational age affects expression, and whether the increase is localized to the rupture site or is membrane-wide.

METHODS

Fetal membranes were collected from 15 pregnancies complicated by preterm PROM and 26 control cases, which delivered at term or preterm without PROM. The preterm PROM cases represented both patients who labored and those who did not. Membrane samples at the rupture site and a remote site (approximately > 5 cm) were analyzed for MMP-9 protein and enzymatic activity by Western blot and gelatin zymography, respectively.

RESULTS

MMP-9 levels in fetal membranes were similar at both the rupture and the remote sites. The highest levels of total MMP-9 protein were found in preterm PROM patients with labor ( < 0.05) and were increased four-fold over protein levels in non-laboring preterm PROM patients delivered by Cesarean section ( < 0.001). In preterm PROM patients without labor, levels of MMP-9 protein were similar to those of non-laboring patients at term and preterm. Zymography correlated with protein results in all membranes.

CONCLUSIONS

Preterm PROM without labor is not associated with increased membrane levels of MMP-9 protein, suggesting that its local elevation does not play a role in early membrane rupture.

摘要

目的

胎膜早破（PROM）占所有早产的30%-40%。本研究的目的是确定基质金属蛋白酶-9（MMP-9）在早产PROM胎儿胎膜中是否升高，分娩或孕周是否影响其表达，以及这种升高是局限于破裂部位还是全膜范围。

方法

收集15例并发早产PROM的妊娠病例及26例足月或早产未发生PROM的对照病例的胎儿胎膜。早产PROM病例包括已分娩和未分娩的患者。分别通过蛋白质印迹法和明胶酶谱法分析破裂部位和远处部位（约>5 cm）的胎膜样本中的MMP-9蛋白和酶活性。

结果

胎膜中MMP-9水平在破裂部位和远处部位相似。在已分娩的早产PROM患者中发现总MMP-9蛋白水平最高（<0.05），比剖宫产分娩的未分娩早产PROM患者的蛋白水平增加了四倍（<0.001）。在未分娩的早产PROM患者中，MMP-9蛋白水平与足月和早产未分娩患者相似。酶谱分析结果与所有胎膜的蛋白质结果相关。