Bryant-Greenwood G D, Yamamoto S Y
Department of Anatomy and Reproductive Biology, University of Hawaii, Manoa, Honolulu, 96822.
Am J Obstet Gynecol. 1995 Jan;172(1 Pt 1):63-70. doi: 10.1016/0002-9378(95)90085-3.
This study was designed to observe the in vivo status of chorion-decidua gene expression for some major metalloproteinases, an inhibitor (tissue inhibitor of metalloproteinase-1), and an activator (tissue plasminogen activator) and the hormone relaxin at accessible time points in the peripartal period.
Chorion-decidua from patients at cesarean section with and without labor and after spontaneous labor and delivery was used for preparation of poly (A)+ ribonucleic acid and quantitative Northern analyses with a series of oligo and complementary deoxyribonucleic acid probes.
In the period designated as the period before parturition, relaxin and matrix metalloproteinase-1 (interstitial collagenase) gene expression were relatively high, reflecting the controlled loss of amniotic collagen necessary for fetal membrane expansion without rupture as the uterine volume increases. When active labor has begun, but before delivery, matrix metalloproteinase-3 (stromelysin) and matrix metalloproteinase-9 (type V collagenase) gene levels significantly increased. After normal spontaneous labor and delivery, tissue plasminogen activator and tissue inhibitor of metalloproteinase-1 messenger ribonucleic acids significantly increased, together with a marginally increased expression of the genes for matrix metalloproteinase-1, matrix metalloproteinase-2 (type IV collagenase), and relaxin.
The expression of the genes for some major collagenolytic enzymes, an inhibitor, activator, and relaxin in the chorion-decidua, is different at the different stages of parturition.
本研究旨在观察围产期可获取时间点绒毛膜 - 蜕膜中一些主要金属蛋白酶、一种抑制剂（金属蛋白酶组织抑制剂 -1）、一种激活剂（组织型纤溶酶原激活剂）以及激素松弛素的体内基因表达状况。
取自剖宫产患者有或无分娩经历以及自然分娩后的绒毛膜 - 蜕膜，用于制备聚腺苷酸核糖核酸，并使用一系列寡核苷酸和互补脱氧核糖核酸探针进行定量Northern分析。
在指定为分娩前的时期，松弛素和基质金属蛋白酶 -1（间质胶原酶）基因表达相对较高，这反映出随着子宫体积增加，胎膜扩张而不破裂所需的羊膜胶原的可控丢失。当活跃分娩开始但尚未分娩时，基质金属蛋白酶 -3（基质溶解素）和基质金属蛋白酶 -9（Ⅴ型胶原酶）基因水平显著升高。正常自然分娩后，组织型纤溶酶原激活剂和金属蛋白酶组织抑制剂 -1信使核糖核酸显著增加，同时基质金属蛋白酶 -1、基质金属蛋白酶 -2（Ⅳ型胶原酶）和松弛素基因的表达略有增加。
绒毛膜 - 蜕膜中一些主要胶原分解酶、一种抑制剂、一种激活剂和松弛素的基因表达在分娩的不同阶段有所不同。
