Vadillo-Ortega F, Hernandez A, Gonzalez-Avila G, Bermejo L, Iwata K, Strauss J F
Departamento de Bioquimica, Instituto Nacional de Perinatologia, Mexico City, Mexico.
Am J Obstet Gynecol. 1996 Apr;174(4):1371-6. doi: 10.1016/s0002-9378(96)70687-7.
It has been suggested that increased matrix metalloproteinases activity promotes the weakening of the amniochorion during normal and premature rupture of membranes. This study was designed to determine whether levels of matrix metalloproteinases and the tissue inhibitor of metalloproteinases-1 in amniotic fluid change in a pattern consistent with this hypothesis.
Gelatinolytic activity, measured by a soluble substrate assay and zymography, and the concentrations of tissue inhibitor of metalloproteinases-1 were estimated in amniotic fluid obtained from (1) normal early gestations, (2) normal term pregnancies with labor, (3) normal term pregnancies without labor, and (4) pregnancies complicated by premature rupture of membranes. The 92 kd type IV collagenase (matrix metalloproteinase-9) was also detected in amniotic fluid by Western blotting.
Matrix metalloproteinase activities were higher in amniotic fluid from normal term pregnancies with labor and pregnancies complicated by premature rupture of membranes than from early pregnancies and term gestations without labor. The amniotic fluid from term pregnancies with labor or pregnancies with premature rupture of membranes contained several gelatinases, as revealed by zymography. The major amniotic fluid gelatinolytic activity in premature rupture of membranes and term pregnancies with labor corresponded to matrix metalloproteinase-9. Tissue inhibitor of metalloproteinases-1 concentrations were highest in early-pregnancy amniotic fluid, followed by term gestation with labor, term gestation without labor, and premature rupture of membranes.
Normal labor and premature rupture of membranes are associated with increased levels of matrix metalloproteinases, particularly matrix metalloproteinase-9 in amniotic fluid. Premature rupture of membranes is associated with reduced levels of tissue inhibitor of metalloproteinases-1. The imbalance between matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 in amniotic fluid may reflect a disorder that promotes premature rupture of membranes.
有人提出，基质金属蛋白酶活性增加会促使羊膜绒毛膜在正常和胎膜早破时变脆弱。本研究旨在确定羊水基质金属蛋白酶水平和金属蛋白酶组织抑制剂-1是否按照与该假设相符的模式变化。
通过可溶性底物分析和酶谱法测定明胶酶活性，并估计从以下来源获取的羊水中金属蛋白酶组织抑制剂-1的浓度：（1）正常早孕；（2）足月分娩的正常妊娠；（3）未分娩的足月正常妊娠；（4）并发胎膜早破的妊娠。还通过蛋白质印迹法在羊水中检测92 kd IV型胶原酶（基质金属蛋白酶-9）。
足月分娩的正常妊娠和并发胎膜早破的妊娠的羊水基质金属蛋白酶活性高于早孕和未分娩的足月妊娠。酶谱法显示，足月分娩的妊娠或胎膜早破妊娠的羊水含有几种明胶酶。胎膜早破和足月分娩妊娠的主要羊水明胶酶活性对应于基质金属蛋白酶-9。金属蛋白酶组织抑制剂-1浓度在早孕羊水中最高，其次是足月分娩妊娠、未分娩的足月妊娠和胎膜早破。
正常分娩和胎膜早破与羊水基质金属蛋白酶水平升高有关，尤其是基质金属蛋白酶-9。胎膜早破与金属蛋白酶组织抑制剂-1水平降低有关。羊水中基质金属蛋白酶与金属蛋白酶组织抑制剂-1之间的失衡可能反映了一种促进胎膜早破的紊乱情况。
