Bagert John D, van Kessel Julia C, Sweredoski Michael J, Feng Lihui, Hess Sonja, Bassler Bonnie L, Tirrell David A
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125.
Department of Molecular and Cellular Biochemistry, Indiana University, Bloomington, IN 47405.
Chem Sci. 2016 Mar 1;7(3):1797-1806. doi: 10.1039/C5SC03340C. Epub 2015 Nov 23.
Bacteria use a process of chemical communication called quorum sensing to assess their population density and to change their behavior in response to fluctuations in the cell number and species composition of the community. In this work, we identified the quorum-sensing-regulated proteome in the model organism by bio-orthogonal non-canonical amino acid tagging (BONCAT). BONCAT enables measurement of proteome dynamics with temporal resolution on the order of minutes. We deployed BONCAT to characterize the time-dependent transition of from individual- to group-behaviors. We identified 176 quorum-sensing-regulated proteins at early, intermediate, and late stages of the transition, and we mapped the temporal changes in quorum-sensing proteins controlled by both transcriptional and post-transcriptional mechanisms. Analysis of the identified proteins revealed 86 known and 90 new quorum-sensing-regulated proteins with diverse functions, including transcription factors, chemotaxis proteins, transport proteins, and proteins involved in iron homeostasis.
细菌利用一种称为群体感应的化学通讯过程来评估其种群密度，并根据群落中细胞数量和物种组成的波动来改变其行为。在这项工作中，我们通过生物正交非天然氨基酸标记（BONCAT）在模式生物中鉴定了群体感应调节的蛋白质组。BONCAT能够以分钟量级的时间分辨率测量蛋白质组动力学。我们利用BONCAT来表征从个体行为到群体行为的时间依赖性转变。我们在转变的早期、中期和后期鉴定了176种群体感应调节蛋白，并绘制了受转录和转录后机制控制的群体感应蛋白的时间变化图谱。对鉴定出的蛋白质的分析揭示了86种已知的和90种新的具有不同功能的群体感应调节蛋白，包括转录因子、趋化蛋白、转运蛋白以及参与铁稳态的蛋白质。
