Shibahara-Sone H, Gomi A, Iino T, Kano M, Nonaka C, Watanabe O, Miyazaki K, Ohkusa T
1 Yakult Central Institute, 5-11 Izumi, Kunitachi, Tokyo 186-8650, Japan.
2 Faculty of Research and Development, Yakult Honsha, 1-19 Higashi Shimbashi, Minato-ku, Tokyo 105-8660, Japan.
Benef Microbes. 2016 Jun;7(3):319-26. doi: 10.3920/BM2015.0138. Epub 2016 Mar 1.
The probiotic strain Bifidobacterium bifidum YIT 10347 has been demonstrated to inhibit Helicobacter pylori activity, prevent injury to the gastric mucosa, and improve general gastric malaise symptoms in H. pylori positive patients. This study aimed to investigate the adhering activity and localisation of B. bifidum YIT 10347 to gastric cells and tissue in vitro, and in human in vivo to clarify the mechanism of its beneficial effects on the stomach. The in vitro study found the adhesion rate of B. bifidum YIT 10347 to human gastric epithelial cells was about 10 times higher than that of lactic acid bacteria and other bifidobacteria. In the human study, 5 H. pylori negative and 12 H. pylori positive subjects ingested milk fermented with B. bifidum YIT 10347. B. bifidum YIT 10347 cells were measured by RT-qPCR for in gastric biopsy samples. Living B. bifidum YIT 10347 cells were detected in the biopsy samples in H. pylori negative subjects (105 cells/g and 104 cells/g at 1 h and 2 h after ingestion, respectively) and H. pylori positive subjects (104 cells/g at 1 h after the ingestion). Moreover, immunostaining analysis of tissue sections found that B. bifidum YIT 10347 cells were located at the interstitial mucin layer of the stomach. These results suggest that cells of probiotic B. bifidum YIT 10347 adhered to the human gastric mucosa in a live state, and that the higher adhering activity of B. bifidum YIT 10347 to the gastric mucosa may be involved in its beneficial effects on the human stomach.
益生菌菌株双歧双歧杆菌YIT 10347已被证明可抑制幽门螺杆菌活性，预防胃黏膜损伤，并改善幽门螺杆菌阳性患者的一般胃部不适症状。本研究旨在调查双歧双歧杆菌YIT 10347在体外对胃细胞和组织以及在人体体内的黏附活性和定位，以阐明其对胃部有益作用的机制。体外研究发现，双歧双歧杆菌YIT 10347对人胃上皮细胞的黏附率比乳酸菌和其他双歧杆菌高约10倍。在人体研究中，5名幽门螺杆菌阴性和12名幽门螺杆菌阳性受试者摄入了用双歧双歧杆菌YIT 10347发酵的牛奶。通过RT-qPCR对胃活检样本中的双歧双歧杆菌YIT 10347细胞进行检测。在幽门螺杆菌阴性受试者的活检样本中检测到活的双歧双歧杆菌YIT 10347细胞（摄入后1小时和2小时分别为105个细胞/克和104个细胞/克）以及幽门螺杆菌阳性受试者的活检样本中（摄入后1小时为104个细胞/克）。此外，组织切片的免疫染色分析发现，双歧双歧杆菌YIT 10347细胞位于胃的间质黏液层。这些结果表明，益生菌双歧双歧杆菌YIT 10347的细胞以活的状态黏附于人胃黏膜，并且双歧双歧杆菌YIT 10347对胃黏膜的较高黏附活性可能与其对人体胃部的有益作用有关。
