Koenka Israel Joel, Sáiz Jorge, Rempel Paul, Hauser Peter C
Department of Chemistry, University of Basel , Spitalstrasse 51, 4056 Basel, Switzerland.
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá , Ctra. Madrid-Barcelona Km 33.6, Alcalá de Henares, 28871, Madrid, Spain.
Anal Chem. 2016 Apr 5;88(7):3761-7. doi: 10.1021/acs.analchem.5b04666. Epub 2016 Mar 10.
A breadboard approach for electrophoretic separations with contactless conductivity detection is presented. This is based on miniature off-the-shelf components such as syringe pumps, valves, and pressure controllers which could be set up in a very compact overall arrangement. It has a high flexibility for different tasks at hand, and the common operations of hydrodynamic injection and capillary flushing are automated. For demonstration of the versatility of the proposition, several very diverse configurations and modes of electrophoresis were successfully implemented, namely, standard capillary zone electrophoresis, pressure assisted zone electrophoresis, the simultaneous separation of cations and anions by dual-capillary zone electrophoresis, the separation of cationic amino acids by isotachophoresis, as well as the separation of small carboxylic acids by gradient elution moving boundary electrophoresis. The system also allows fast separations, as demonstrated by the analysis of six inorganic cations within 35 s. The approach addresses respective limitations of either conventional capillary electrophoresis instruments as well as electrophoretic lab-on-chip devices, while maintaining a performance in terms of detection limits and reproducibility comparable to standard instrumentation.
本文介绍了一种采用非接触式电导检测的面包板电泳分离方法。该方法基于微型现成组件，如注射泵、阀门和压力控制器，这些组件可以以非常紧凑的整体布局进行设置。它对于手头的不同任务具有高度的灵活性，并且流体动力进样和毛细管冲洗等常见操作是自动化的。为了证明该方案的多功能性，成功实现了几种非常不同的电泳配置和模式，即标准毛细管区带电泳、压力辅助区带电泳、双毛细管区带电泳同时分离阳离子和阴离子、等速电泳分离阳离子氨基酸以及梯度洗脱移动边界电泳分离小羧酸。该系统还允许快速分离，如在35秒内分析六种无机阳离子所示。该方法解决了传统毛细管电泳仪器和电泳芯片实验室设备各自的局限性，同时在检测限和重现性方面保持了与标准仪器相当的性能。
