Zhang Ying, Feng Gui-Hai, Xu Kai, Wang Libin, Cui Peng, Li Yuhuan, Wang Chenxin, Teng Fei, Hao Jie, Wan Hai-Feng, Tan Yuanqing, Wang Xiu-Jie, Zhou Qi
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Key Laboratory of Genetic Network Biology, Collaborative Center for Genetics and Development, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Sci Rep. 2016 Mar 1;6:22380. doi: 10.1038/srep22380.
To precisely determine the type and status of cells is an important prerequisite for basic researches and regenerative medicine involving stem cells or differentiated cells. However, the traditional destructive cell status examination methods have many limitations, mainly due to the heterogeneity of cells under the reprogramming or differentiation/trans-differentiation process. Here we present a new method to non-destructively determine the pluripotent level of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), or the types of differentiated cells. The method is achieved by examining the expression profiles of microRNAs (miRNAs) in cell culture medium, which show consistent abundance trend as those of the cellular miRNAs. Therefore, the method enables status examination and afterward application being achieved on the same population of cells, which will greatly facilitate cell reprogramming or differentiation/trans-differentiation related based research and clinical therapy.
精确确定细胞的类型和状态是涉及干细胞或分化细胞的基础研究和再生医学的重要前提。然而，传统的破坏性细胞状态检测方法存在许多局限性，这主要是由于细胞在重编程或分化/转分化过程中的异质性所致。在此，我们提出一种新方法，可无损地确定胚胎干细胞（ESC）和诱导多能干细胞（iPSC）的多能水平，或分化细胞的类型。该方法通过检测细胞培养基中微小RNA（miRNA）的表达谱来实现，这些miRNA的丰度趋势与细胞内miRNA一致。因此，该方法能够在同一群细胞上实现状态检测及后续应用，这将极大地促进基于细胞重编程或分化/转分化的相关研究及临床治疗。
