Ono B, Tanaka M, Awano I, Okamoto F, Satoh R, Yamagishi N, Ishino-Arao Y
Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Curr Genet. 1989 Dec;16(5-6):323-30. doi: 10.1007/BF00340710.
Ten dominant omnipotent suppressors of Saccharomyces cerevisiae, which were previously shown to be different from SUP46, have been examined. Nine are mapped in a region between lys5 and cyh2 on the left arm of chromosome VII. These suppressors, like SUP46, manifest sensitivity to increased temperature and the antibiotics paromomycin and hygromycin B. In addition, they have an identical action spectrum. These results strongly suggest that they are allelic to each other and they are designated SUP138. The tenth is mapped to a position between his1 and arg6 on the right arm of chromosome V. This suppressor, named SUP139, does not manifest temperature sensitivity nor antibiotic sensitivity. SUP139 and SUP138, which are clearly distinguished by means of action spectrum, act on much fewer nonsense mutations than SUP46. It is now clear that dominant omnipotent suppressors arising at a single locus are homogeneous and that their efficiency is locus-dependent. The order of efficiency is SUP46 greater than SUP138 greater than SUP139.
对酿酒酵母的十个主要全能抑制子进行了检测，先前已证明它们与SUP46不同。其中九个定位在第七条染色体左臂上lys5和cyh2之间的区域。这些抑制子与SUP46一样，对温度升高以及抗生素巴龙霉素和潮霉素B表现出敏感性。此外，它们具有相同的作用谱。这些结果强烈表明它们彼此等位，被命名为SUP138。第十个定位在第五条染色体右臂上his1和arg6之间的位置。这个抑制子名为SUP139，不表现出温度敏感性和抗生素敏感性。SUP139和SUP138通过作用谱明显区分，它们作用于比SUP46少得多的无义突变。现在很清楚，在单个位点产生的显性全能抑制子是同质的，并且它们的效率取决于位点。效率顺序为SUP46大于SUP138大于SUP139。
