Ono B, Chernoff Y O, Ishino-Arao Y, Yamagishi N, Shinoda S, Inge-Vechtomov S G
Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Curr Genet. 1991 Apr;19(4):243-8. doi: 10.1007/BF00355049.
Chromosomal omnipotent suppressor mutations recovered in psi+ strains of Saccharomyces cerevisiae were brought into psi- cytoplasm. SUP46, SUP138 and SUP139 acted as dominant omnipotent suppressors in the psi- cytoplasm though their suppressor activity was substantially reduced. SUP46 and SUP138 conferred recessive thermosensitivity and antibiotic sensitivity in psi- cytoplasm as in psi+ cytoplasm. On the other hand, sup111 through sup115, which acted as recessive omnipotent suppressors in the psi+ cytoplasm, manifested no, or very low, suppressor activity in the psi- cytoplasm. They, however, still enhanced the efficiency of the SUP29 tRNA suppressor in psi- cytoplasm. A multicopy plasmid carrying the wild-type SUP35 gene enhanced the efficiency of sup111 in psi- cytoplasm.
在酿酒酵母的ψ⁺菌株中恢复的染色体全能抑制突变被导入到ψ⁻细胞质中。SUP46、SUP138和SUP139在ψ⁻细胞质中作为显性全能抑制因子起作用，尽管它们的抑制活性大幅降低。SUP46和SUP138在ψ⁻细胞质中与在ψ⁺细胞质中一样，赋予隐性温度敏感性和抗生素敏感性。另一方面，在ψ⁺细胞质中作为隐性全能抑制因子起作用的sup111至sup115，在ψ⁻细胞质中表现出无或非常低的抑制活性。然而，它们仍然提高了ψ⁻细胞质中SUP29 tRNA抑制因子的效率。携带野生型SUP35基因的多拷贝质粒提高了ψ⁻细胞质中sup111的效率。
