Kalaiselvan A, Anand T, Gokulakrishnan K, Kamaraj M C, Velavan S
Centre for Research and Development, Marudupandiyar College, Thanjavur, Tamil Nadu, India.
Department of Chemistry, PRIST University, Thanjavur, Tamil Nadu, India.
Pharmacogn Mag. 2015 Oct;11(Suppl 3):S496-500. doi: 10.4103/0973-1296.168981.
The modulations of glucose-metabolizing enzyme activities play a vital rolein the depletion of energy metabolism and leads to inhibition of cancer growth.
To find the effect of shorearobusta bark extract on glucose-metbolizing enzymes in diethylnitrosamine (DEN) induced hepatocellular carcinoma rats.
Biochemical evaluation of glucose metabolizing enzyme were done in before and after shorearobusta bark extract (500mg/kg) treatment in DEN induced rats.
A significant increasein the activities of the key glycolytic enzymes viz., hexokinase and phosphoglucoisomerase, with a significant decrease in the gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatasewere observed in HCC bearing rats, when compared with the control. Administration of shorearobusta extract caused a significant decrease in theactivities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values.
The current findings suggest that the S. robusta extract has a definite modulating role on the key enzymes ofglucose-metabolism in HCC. The modulatory effect may be due to the phytoactive constituents present in the extract of S. robusta.
Administration of shorea robusta bark extract caused a significant decrease in the activities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values. The S. robusta extract has modulatory activity on the carbohydrate metabolism in DEN-induced HCC bearing rats through a mechanism that which does not provoke any acute biochemical disturbances in the metabolic pathways of glycolysis and gluconeogenesis. The modulatory effect of S. robusta extract may be attributed to the presence of active compounds such as polyphenols and flavonoids. Abbreviations used: HCC: Hepatocellular Carcinoma, SRBE: Shorearobusta bark extract; HEX: Hexokinase; PGI: Phosphoglucoisomerase; DEN: Diethylnitrosamine.
葡萄糖代谢酶活性的调节在能量代谢耗竭中起关键作用，并导致癌症生长受到抑制。
研究娑罗双树皮提取物对二乙基亚硝胺（DEN）诱导的肝癌大鼠葡萄糖代谢酶的影响。
对DEN诱导的大鼠在给予娑罗双树皮提取物（500mg/kg）治疗前后进行葡萄糖代谢酶的生化评估。
与对照组相比，在荷肝癌大鼠中观察到关键糖酵解酶即己糖激酶和磷酸葡萄糖异构酶的活性显著增加，而糖异生酶葡萄糖-6-磷酸酶和果糖-1,6-二磷酸酶的活性显著降低。给予娑罗双提取物导致糖酵解酶活性显著降低，糖异生酶活性增加至接近正常水平。
目前的研究结果表明，娑罗双提取物对肝癌中葡萄糖代谢的关键酶具有明确的调节作用。这种调节作用可能归因于娑罗双提取物中存在的植物活性成分。
给予娑罗双树皮提取物导致糖酵解酶活性显著降低，糖异生酶活性增加至接近正常水平。娑罗双提取物通过一种不会在糖酵解和糖异生代谢途径中引发任何急性生化紊乱的机制，对DEN诱导的荷肝癌大鼠的碳水化合物代谢具有调节活性。娑罗双提取物的调节作用可能归因于多酚和黄酮类等活性化合物的存在。使用的缩写：HCC：肝细胞癌，SRBE：娑罗双树皮提取物；HEX：己糖激酶；PGI：磷酸葡萄糖异构酶；DEN：二乙基亚硝胺。
