Novin M Ghaffari, Allahveisi A, Noruzinia M, Farhadifar F, Yousefian E, Fard A Dehghani, Salimi M
Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran.
Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Balkan J Med Genet. 2015 Dec 30;18(1):39-46. doi: 10.1515/bjmg-2015-0004. eCollection 2015 Jun.
In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during oocyte maturation. The purpose of this study was to identify the relationship between transcript expression levels of the mitochondrial encoded gene (MT-CO1) and two nuclear encoded genes, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in various stages of human oocyte maturation. Nine consenting patients, age 21-35 years old, with male factors were selected for ovarian stimulation and intracytoplasmic sperm injection (ICSI) procedures. mRNA levels of mitochondrial-related genes were performed by singlecell TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant relationship between the relative expression levels in germinal vesicle (GV) stage oocytes (p = 0.62). On the contrary, a significant relationship was seen between the relative expression levels of TFAM and NRF1 and the MT-CO1 genes at the stages of metaphase I (MI) and MII (p = 0.03 and p = 0.002). A relationship exists between the transcript expression levels of TFAM and NRF1, and MT-CO1 genes in various stages of human oocyte maturation.
在一些女性不孕症病例中，人类卵母细胞在达到减数分裂中期II（MII）阶段时无法成熟。线粒体在卵母细胞成熟过程中起着重要作用。在卵母细胞中复制的大量线粒体DNA（mtDNA）对于在卵母细胞成熟期间提供三磷酸腺苷（ATP）至关重要。本研究的目的是确定线粒体编码基因（MT-CO1）与两个核编码基因，即核呼吸因子1（NRF1）和线粒体转录因子A（TFAM）在人类卵母细胞成熟各个阶段的转录表达水平之间的关系。选择了9名年龄在21 - 35岁、因男性因素导致不孕的患者，进行卵巢刺激和卵胞浆内单精子注射（ICSI）手术。通过单细胞TaqMan®定量实时聚合酶链反应（qRT-PCR）检测线粒体相关基因的mRNA水平。在生发泡（GV）期卵母细胞中，相对表达水平之间没有显著关系（p = 0.62）。相反，在减数分裂中期I（MI）和MII阶段，TFAM和NRF1与MT-CO1基因的相对表达水平之间存在显著关系（p = 0.03和p = 0.002）。在人类卵母细胞成熟的各个阶段，TFAM和NRF1与MT-CO1基因的转录表达水平之间存在关联。
