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玉米淀粉制成的膳食纤维制剂对肥胖儿童粪便样本中厚壁菌门、拟杆菌门和放线菌门某些细菌生长及活性的影响

Effects of dietary fiber preparations made from maize starch on the growth and activity of selected bacteria from the Firmicutes, Bacteroidetes, and Actinobacteria phyla in fecal samples from obese children.

作者信息

Barczynska Renata, Slizewska Katarzyna, Litwin Mieczyslaw, Szalecki Mieczyslaw, Kapusniak Janusz

机构信息

Institute of Chemistry, Environmental Protection and Biotechnology, Jan Dlugosz University in Czestochowa, Czestochowa, Poland.

Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Technical University of Lodz, Łódź, Poland.

出版信息

Acta Biochim Pol. 2016;63(2):261-6. doi: 10.18388/abp.2015_1068. Epub 2016 Feb 29.

Abstract

Currently, there is a search for substances that would be very well tolerated by an organism and which could contribute to the activation of the growth of Bacteroidetes and Actinobacteria strains, with simultaneous inhibition of the growth of Firmicutes. High expectations in this regard are raised with the use of fiber preparations from starch - resistant corn dextrins, branched dextrins, resistant maltodextrins and soluble corn fiber. In this paper, the influence of fiber preparations made from corn starch was evaluated on growth and activity of Bacteroidetes, Actinobacteria and Firmicutes strains isolated from obese children. It was demonstrated that in the stool of obese children Firmicutes strains predominate, while Bacteroidetes and Actinobacteria strains were in the minority. A supplementation of fecal culture with fiber preparations did not cause any significant changes in the number of strains of Bacteroidetes and Firmicutes. Addition of fiber preparations to the fecal samples of obese children increased the amount of short-chain fatty acids, especially acetic (p < 0.01), propionic, butyric (p = 0.05) and lactic acid (p < 0.01).

摘要

目前,人们正在寻找生物体耐受性良好且有助于拟杆菌属和放线菌属菌株生长激活,同时抑制厚壁菌门生长的物质。在这方面,使用来自抗性玉米糊精、支链糊精、抗性麦芽糊精和可溶性玉米纤维的纤维制剂引发了人们的高度期望。本文评估了由玉米淀粉制成的纤维制剂对从肥胖儿童中分离出的拟杆菌属、放线菌属和厚壁菌门菌株的生长及活性的影响。结果表明,在肥胖儿童的粪便中,厚壁菌门菌株占主导,而拟杆菌属和放线菌属菌株占少数。用纤维制剂补充粪便培养物并未导致拟杆菌属和厚壁菌门菌株数量发生任何显著变化。向肥胖儿童的粪便样本中添加纤维制剂增加了短链脂肪酸的量,尤其是乙酸(p < 0.01)、丙酸、丁酸(p = 0.05)和乳酸(p < 0.01)。

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