Department of Pediatric Research Institute, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics Metabolism and Inflammatory Disease, Children's Hospital of Chongqing Medical University, Chongqing, China.
Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing, China.
BMC Microbiol. 2024 Aug 24;24(1):311. doi: 10.1186/s12866-024-03461-8.
To explore the distribution and differences in the intestinal microbiota in girls with obesity-related precocious puberty and the relationship between intestinal microbiota and obesity-related precocious puberty.
16 S rRNA gene amplicons from fecal samples from girls with precocious puberty and obesity-complicated precocious puberty and healthy children were sequenced to define microbial taxa.
The α- and β-diversity indices of the microbiome significantly differed among the three groups. At the phylum level, the proportions of Firmicutes, Actinobacteriota, Bacteroidota, Bacteria, Campylobacterota, and Acidobacteriota were different. At the genus level, there were differences in Bifidobacterium, Bacteroides, Anaerostipes, Fusicatenibacter, Klebsiella, Lachnospiraceae, ErysipelotrichaceaeUCG-003, Prevotella9, Ruminococcus gnavus group, and Lachnoclostridium. Additionally, Bifidobacterium, Anaerostipes, Bacteroides, Candidatus Microthrix, Eubacterium hallii group, Klebsiella, and Erysipelotrichaceae UCG-003 were identified as bacterial biomarkers by LEfSe. Furthermore, Sellimonas, Intestinibacter, Anaerostipes, Ruminococcus gnavus group, and Oscillibacter were identified as the differential biomarkers by random forest. A receiver operating characteristic (ROC) curve was used to evaluate the biomarkers with high predictive value for obesity-related precocious puberty. Spearman correlation analysis confirmed that Anaerostipes levels were negatively correlated with body weight, body mass index (BMI), bone age, luteinizing hormone, follicle-stimulating hormone, and estradiol.
There was a significant correlation between obesity-associated precocious puberty and gut microbiota, especially the functional characteristics of the microbiome and its interactions, which can provide a theoretical basis for the clinical intervention of obesity and precocious puberty through the microbiome.
探讨肥胖相关性性早熟女童肠道微生物群的分布和差异，以及肠道微生物群与肥胖相关性性早熟的关系。
对性早熟女童和肥胖伴性早熟女童及健康儿童的粪便样本进行 16S rRNA 基因扩增子测序，以确定微生物分类群。
三组间微生物组的α-和β多样性指数差异有统计学意义。在门水平上，厚壁菌门、放线菌门、拟杆菌门、细菌门、弯曲杆菌门和酸杆菌门的比例不同。在属水平上，双歧杆菌、拟杆菌、厌氧棒状杆菌、Fusicatenibacter、克雷伯菌、lachnospiraceae、ErysipelotrichaceaeUCG-003、普雷沃氏菌 9、Ruminococcus gnavus 组和lachnoclostridium 存在差异。此外，双歧杆菌、厌氧棒状杆菌、拟杆菌、Candidatus Microthrix、Eubacterium hallii 组、克雷伯菌和 Erysipelotrichaceae UCG-003 通过 LEfSe 被鉴定为细菌生物标志物。此外，Sellimonas、Intestinibacter、厌氧棒状杆菌、Ruminococcus gnavus 组和 Oscillibacter 通过随机森林被鉴定为差异生物标志物。使用受试者工作特征（ROC）曲线评估对肥胖相关性性早熟具有高预测价值的生物标志物。Spearman 相关性分析证实，厌氧棒状杆菌水平与体重、体重指数（BMI）、骨龄、黄体生成素、卵泡刺激素和雌二醇呈负相关。
肥胖相关性性早熟与肠道微生物群密切相关，尤其是微生物群的功能特征及其相互作用，这可为通过微生物群对肥胖和性早熟进行临床干预提供理论依据。
